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Original Article
- Clinical Study
- Leu72Met and Other Intronic Polymorphisms in the GHRL and GHSR Genes Are Not Associated with Type 2 Diabetes Mellitus, Insulin Resistance, or Serum Ghrelin Levels in a Saudi Population
- Faris Elbahi Joatar, Ali Ahmed Al Qarni, Muhalab E. Ali, Abdulaziz Al Masaud, Abdirashid M. Shire, Nagalla Das, Khalid Gumaa, Hayder A. Giha
- Endocrinol Metab. 2017;32(3):360-369. Published online September 18, 2017
- DOI: https://doi.org/10.3803/EnM.2017.32.3.360
- 4,476 View
- 49 Download
- 9 Web of Science
- 11 Crossref
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Abstract
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PubReader Background Ghrelin (GHRL), a gastric peptide encoded by the
GHRL gene, is known to be involved in energy homeostasis via its G protein receptor, encoded by the growth hormone secretagogue receptor (GHSR ) gene. Some studies have shown associations between plasma GHRL levels andGHRL single-nucleotide polymorphisms (SNPs), namely the Leu72Met polymorphism (rs696217 TG), with type 2 diabetes mellitus (T2DM) and insulin resistance (IR), while others have not. The controversies in these associations raise the issue of ‘which SNPs in which populations.’ The aim of this study was to investigate whether SNPs inGHRL and/orGHSR genes were associated with T2DM, IR, or plasma GHRL levels among Arab Saudis.Methods Blood was collected from 208 Saudi subjects with (
n =107) and without (n =101) T2DM. DNA samples from these subjects were analyzed by real-time polymerase chain reaction to genotype five intronic SNPs in theGHRL (rs696217 TG, rs27647 CT, rs2075356 CT, and rs4684677 AT) andGHSR (rs509030 GC) genes. In addition, plasma GHRL levels were measured by a radioimmunoassay.Results None of the SNPs were associated with T2DM, IR, or plasma GHRL levels. The frequencies of the alleles, genotypes, and haplotypes of the five SNPs were comparable between the T2DM patients and the non-diabetic subjects. A large number of the
GHRL haplotypes indicates the molecular heterogeneity of the preproghrelin gene in this region.Conclusion Neither the Leu72Met polymorphism nor the other intronic GHRL and
GHSR SNPs were associated with T2DM, IR, or GHRL levels. Further investigations should be carried out to explain the molecular basis of the association of the GHRL peptide with T2DM and IR.-
Citations
Citations to this article as recorded by
- Relationship between single nucleotide polymorphism studies in ghrelin gene with obesity subjects
May Salem Al-Nbaheen
Journal of King Saud University - Science.2023; 35(1): 102393. CrossRef - Leu72Met Polymorphism in Ghrelin Gene: A Potential Risk Factor for Hypertension in Type 2 Diabetes Patients
Monika Buraczynska, Jakub Golacki, Wojciech Zaluska
Diabetes, Metabolic Syndrome and Obesity.2023; Volume 16: 557. CrossRef - Асоціації варіантів гена GHRL із розвитком ожиріння та метаболічних порушень у дітей
A. Abaturov, A. Nikulina
CHILD`S HEALTH.2023; 18(4): 255. CrossRef - Effect of the GHRL gene (rs696217) polymorphism on the metabolic disorders in patients with obesity in the Ukrainian population
Andrii Prodan, Ihor Dzubanovsky, Oleksandr Kamyshnyi, Natalia Melnyk, Stepan Grytsenko, Stanislava Voloshyn
Endocrine Regulations.2023; 57(1): 173. CrossRef - Impact of gene polymorphism of glutathione S-transferase and ghrelin as a risk factor in Egyptian women with gestational diabetes mellitus
Mai M. Madkour, Afaf M. El-Said, Abd El-Aziz A. El-Refaey, Abd El-Aziz F. Abd El-Aziz, Fardous F. El-Senduny
Egyptian Journal of Medical Human Genetics.2022;[Epub] CrossRef -
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Hayder A. Giha, Faris E. Joatar, Dhuha M. B. AlDehaini, Zainab H. A. Malalla, Muhalab E. Ali, Ali A. Al Qarni
Hormone Molecular Biology and Clinical Investigation.2022; 43(3): 297. CrossRef - Investigation of GHRL (rs4684677), FTO (rs8044769) and PGC1Α (rs8192678) polymorphisms in type 2 diabetic Turkish population
Osman Oğuz, Arezoo Gheybi, Zeliha Doğan, Feray Akbaş, Ümit Zeybek, Arzu Ergen
Turkish Journal of Biochemistry.2022; 47(5): 564. CrossRef - Grelin ve Grelin Reseptörü Polimorfizmlerinin Tip 2 Diyabetle İlişkisi
Esma SELÇUK, Uğur ŞAHİN, Didem ÖZKAHRAMAN, Mustafa CALAPOĞLU, Nilüfer ŞAHİN CALAPOĞLU
Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi.2022; 13(2): 218. CrossRef - Variation analysis of Ghrelin gene in Chinese patients with obesity, having polycystic ovarian syndrome
Xiaomeng Wang, Fengxiang Qu, Chunlian Wang, Yan Wang, Dan Wang, Min Zhao, Xiangbing Yun, Qingmei Zheng, Lin Xu
Gynecological Endocrinology.2020; 36(7): 594. CrossRef - The flavonoid baicalin improves glucose metabolism by targeting the PH domain of AKT and activating AKT/GSK3β phosphorylation
Shengnan Yang, Yuan Zhang, Fukui Shen, Xiaoyao Ma, Man Zhang, Yuanyuan Hou, Gang Bai
FEBS Letters.2019; 593(2): 175. CrossRef - Ethnicity-Specific Association Between Ghrelin Leu72Met Polymorphism and Type 2 Diabetes Mellitus Susceptibility: An Updated Meta-Analysis
Rong Huang, Sai Tian, Rongrong Cai, Jie Sun, Yanjue Shen, Shaohua Wang
Frontiers in Genetics.2018;[Epub] CrossRef
- Relationship between single nucleotide polymorphism studies in ghrelin gene with obesity subjects
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