Endocrinology and Metabolism

Original Articles

Endocrine Research
The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes: Cheol Ryong Ku, Yoon Hee Cho, Zhen-Yu Hong, Ha Lee, Sue Ji Lee, Seung-soo Hong, Eun Jig Lee; Endocrinol Metab. 2016;31(2):328-335. Published online April 8, 2016; DOI: https://doi.org/10.3803/EnM.2016.31.2.328

4,168 View
50 Download
25 Web of Science
25 Crossref

Background

Resveratrol (RSV) is a polyphenolic phytoalexin that has many effects on metabolic diseases such as diabetes and obesity. Given the importance of brown adipose tissue (BAT) for energy expenditure, we investigated the effects of RSV on brown adipocytes.

Methods

For the in vitro study, interscapular BAT was isolated from 7-week-old male Sprague Dawley rats. For the in vivo study, 7-week-old male Otsuka Long Evans Tokushima Fatty (OLETF) rats were divided into four groups and treated for 27 weeks with: standard diet (SD); SD+RSV (10 mg/kg body weight, daily); high fat diet (HFD); HFD+RSV. RSV was provided via oral gavage once daily during the in vivo experiments.

Results

RSV treatment of primary cultured brown preadipocytes promoted mitochondrial activity, along with over-expression of estrogen receptor α (ER-α). In OLETF rats, both HFD and RSV treatment increased the weight of BAT and the differentiation of BAT. However, only RSV increased the mitochondrial activity and ER-α expression of BAT in the HFD-fed group. Finally, RSV improved the insulin sensitivity of OLETF rats by increasing the mitochondrial activity of BAT, despite having no effects on white adipocytes and muscles in either diet group.

Conclusion

RSV could improve insulin resistance, which might be associated with mitochondrial activity of brown adipocyte. Further studies evaluating the activity of RSV for both the differentiation and mitochondrial activity of BAT could be helpful in investigating the effects of RSV on metabolic parameters.

Citations

Citations to this article as recorded by

Natural Bioactive Compounds from Foods Inhibited Pigmentation Especially Potential Application of Fucoxanthin to Chloasma: a Mini-Review
Yida Wang, Hang Qi
Food Reviews International.2024; 40(1): 20. CrossRef
Resveratrol combats chronic diseases through enhancing mitochondrial quality
Weichu Tao, Hu Zhang, Xia Jiang, Ning Chen
Food Science and Human Wellness.2024; 13(2): 597. CrossRef
The Potential to Fight Obesity with Adipogenesis Modulating Compounds
Jiaqi Zhao, Ailin Zhou, Wei Qi
International Journal of Molecular Sciences.2022; 23(4): 2299. CrossRef
Macrophage and Adipocyte Mitochondrial Dysfunction in Obesity-Induced Metabolic Diseases
Liwen Wang, Jie Hu, Haiyan Zhou
The World Journal of Men's Health.2021; 39(4): 606. CrossRef
Precision Nutrition to Activate Thermogenesis as a Complementary Approach to Target Obesity and Associated-Metabolic-Disorders
Marina Reguero, Marta Gómez de Cedrón, Sonia Wagner, Guillermo Reglero, José Carlos Quintela, Ana Ramírez de Molina
Cancers.2021; 13(4): 866. CrossRef
Natural Antioxidant Application on Fat Accumulation: Preclinical Evidence
Proshanta Roy, Daniele Tomassoni, Enea Traini, Ilenia Martinelli, Maria Vittoria Micioni Di Bonaventura, Carlo Cifani, Francesco Amenta, Seyed Khosrow Tayebati
Antioxidants.2021; 10(6): 858. CrossRef
Activation of Brown Adipose Tissue and Promotion of White Adipose Tissue Browning by Plant-based Dietary Components in Rodents: A Systematic Review
Francisco J Osuna-Prieto, Borja Martinez-Tellez, Antonio Segura-Carretero, Jonatan R Ruiz
Advances in Nutrition.2021; 12(6): 2147. CrossRef
Role of Dietary Polyphenols in Adipose Tissue Browning: A Narrative Review
Juan Salazar, Clímaco Cano, José L. Pérez, Ana Castro, María P. Díaz, Bermary Garrido, Rubén Carrasquero, Maricarmen Chacín, Manuel Velasco, Luis D´Marco, Joselyn Rojas-Quintero, Valmore Bermúdez
Current Pharmaceutical Design.2020; 26(35): 4444. CrossRef
Brown and Brite: The Fat Soldiers in the Anti-obesity Fight
Shireesh Srivastava, Richard L. Veech
Frontiers in Physiology.2019;[Epub] CrossRef
Effect of resveratrol on adipokines and myokines involved in fat browning: Perspectives in healthy weight against obesity
Oh Yoen Kim, Ji Yeon Chung, Juhyun Song
Pharmacological Research.2019; 148: 104411. CrossRef
Ginsenoside Rb2 Alleviates Obesity by Activation of Brown Fat and Induction of Browning of White Fat
Yilian Hong, Yi Lin, Qiya Si, Lijuan Yang, Weisong Dong, Xuejiang Gu
Frontiers in Endocrinology.2019;[Epub] CrossRef
Programming of the Beige Phenotype in White Adipose Tissue of Adult Mice by Mild Resveratrol and Nicotinamide Riboside Supplementations in Early Postnatal Life
Alba Serrano, Madhu Asnani-Kishnani, Ana María Rodríguez, Andreu Palou, Joan Ribot, María Luisa Bonet
Molecular Nutrition & Food Research.2018;[Epub] CrossRef
Effects of Polyphenols on Thermogenesis and Mitochondrial Biogenesis
Tanila Wood dos Santos, Quélita Cristina Pereira, Lucimara Teixeira, Alessandra Gambero, Josep A. Villena, Marcelo Lima Ribeiro
International Journal of Molecular Sciences.2018; 19(9): 2757. CrossRef
Programming mediated by fatty acids affects uncoupling protein 1 (UCP-1) in brown adipose tissue
Perla P. Argentato, Helena de Cássia César, Débora Estadella, Luciana P. Pisani
British Journal of Nutrition.2018; 120(6): 619. CrossRef
Effects of Genistein on Differentiation and Viability of Human Visceral Adipocytes
Elena Grossini, Serena Farruggio, Giulia Raina, David Mary, Giacomo Deiro, Sergio Gentilli
Nutrients.2018; 10(8): 978. CrossRef
A comprehensive review of the health perspectives of resveratrol
Abdur Rauf, Muhammad Imran, Hafiz Ansar Rasul Suleria, Bashir Ahmad, Dennis G. Peters, Mohammad S. Mubarak
Food & Function.2017; 8(12): 4284. CrossRef
The Role of Circulating Slit2, the One of the Newly Batokines, in Human Diabetes Mellitus
Yea Eun Kang, Sorim Choung, Ju Hee Lee, Hyun Jin Kim, Bon Jeong Ku
Endocrinology and Metabolism.2017; 32(3): 383. CrossRef
A nutritional perspective on UCP1-dependent thermogenesis
M. Luisa Bonet, Josep Mercader, Andreu Palou
Biochimie.2017; 134: 99. CrossRef
The Beneficial Effects of Quercetin, Curcumin, and Resveratrol in Obesity
Yueshui Zhao, Bo Chen, Jing Shen, Lin Wan, Yinxin Zhu, Tao Yi, Zhangang Xiao
Oxidative Medicine and Cellular Longevity.2017; 2017: 1. CrossRef
Articles inEndocrinology and Metabolismin 2016
Won-Young Lee
Endocrinology and Metabolism.2017; 32(1): 62. CrossRef
Antiobesity effects of resveratrol: which tissues are involved?
Alfredo Fernández‐Quintela, Iñaki Milton‐Laskibar, Marcela González, Maria P. Portillo
Annals of the New York Academy of Sciences.2017; 1403(1): 118. CrossRef
Resveratrol attenuates triglyceride accumulation associated with upregulation of Sirt1 and lipoprotein lipase in 3T3-L1 adipocytes
Haruki Imamura, Daiji Nagayama, Noriko Ishihara, Syo Tanaka, Rena Watanabe, Yasuhiro Watanabe, Yuta Sato, Takashi Yamaguchi, Noriko Ban, Hidetoshi Kawana, Masahiro Ohira, Kei Endo, Atsuhito Saiki, Kohji Shirai, Ichiro Tatsuno
Molecular Genetics and Metabolism Reports.2017; 12: 44. CrossRef
Resveratrol has dose-dependent effects on DNA fragmentation and mitochondrial activity of ovine secondary follicles cultured in vitro
T.J.S. Macedo, V.R.P. Barros, A.P.O. Monte, B.B. Gouveia, M.É.S. Bezerra, A.Y.P. Cavalcante, R.S. Barberino, V.G. Menezes, M.H.T. Matos
Zygote.2017; 25(4): 434. CrossRef
Response: The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes (Endocrinol Metab2016;31:328-35, Cheol Ryong Ku et al.)
Cheol Ryong Ku, Eun Jig Lee
Endocrinology and Metabolism.2016; 31(3): 482. CrossRef
Letter: The Effects of High Fat Diet and Resveratrol on Mitochondrial Activity of Brown Adipocytes (Endocrinol Metab2016;31:328-35, Cheol Ryong Ku et al.)
Ji-Young Cha
Endocrinology and Metabolism.2016; 31(3): 480. CrossRef

Obesity and Metabolism
Effect of Resveratrol, a SIRT1 Activator, on the Interactions of the CLOCK/BMAL1 Complex: Insung Park, Yool Lee, Hee-Dae Kim, Kyungjin Kim; Endocrinol Metab. 2014;29(3):379-387. Published online September 25, 2014; DOI: https://doi.org/10.3803/EnM.2014.29.3.379

3,543 View
35 Download
24 Web of Science
22 Crossref

Abstract PDF PubReader

Background

In mammals, the CLOCK/BMAL1 heterodimer is a key transcription factor complex that drives the cyclic expression of clock-controlled genes involved in various physiological functions and behavioral consequences. Recently, a growing number of studies have reported a molecular link between the circadian clock and metabolism. In the present study, we explored the regulatory effects of SIRTUIN1 (SIRT1), an NAD⁺-dependent deacetylase, on CLOCK/BMAL1-mediated clock gene expression.

Methods

To investigate the interaction between SIRT1 and CLOCK/BMAL1, we conducted bimolecular fluorescence complementation (BiFC) analyses supplemented with immunocytochemistry assays. BiFC experiments employing deletion-specific mutants of BMAL1 were used to elucidate the specific domains that are necessary for the SIRT1-BMAL1 interaction. Additionally, luciferase reporter assays were used to delineate the effects of SIRT1 on circadian gene expression.

Results

BiFC analysis revealed that SIRT1 interacted with both CLOCK and BMAL1 in most cell nuclei. As revealed by BiFC assays using various BMAL1 deletion mutants, the PAS-B domain of BMAL1 was essential for interaction with SIRT1. Activation of SIRT1 with resveratrol did not exert any significant change on the interaction with the CLOCK/BMAL1 complex. However, promoter analysis using Per1-Luc and Ebox-Luc reporters showed that SIRT1 significantly downregulated both promoter activities. This inhibitory effect was intensified by treatment with resveratrol, indicating a role for SIRT1 and its activator in CLOCK/BMAL1-mediated transcription of clock genes.

Conclusion

These results suggest that SIRT1 may form a regulatory complex with CLOCK/BMAL1 that represses clock gene expression, probably via deacetylase activity.

Citations

Citations to this article as recorded by

Effects of (poly)phenols on circadian clock gene-mediated metabolic homeostasis in cultured mammalian cells: a scoping review.
Noha Sulaimani, Michael J. Houghton, Maxine P. Bonham, Gary Williamson
Advances in Nutrition.2024; : 100232. CrossRef
Synchronization of Fibroblasts Ex Vivo in Psychopharmacology
Frank Faltraco, Adriana Uzoni, Liliia Shevchuk, Johannes Thome, Denise Palm
Pharmacopsychiatry.2023; 56(03): 101. CrossRef
Resveratrol as a circadian clock modulator: mechanisms of action and therapeutic applications
Weronika Spaleniak, Muriel Cuendet
Molecular Biology Reports.2023; 50(7): 6159. CrossRef
New insights into the mechanisms of diabetic kidney disease: Role of circadian rhythm and Bmal1
Zhimei Peng, Yanting Liang, Xueying Liu, Jie Shao, Nan Hu, Xinzhou Zhang
Biomedicine & Pharmacotherapy.2023; 166: 115422. CrossRef
The circadian machinery links metabolic disorders and depression: A review of pathways, proteins and potential pharmacological interventions
Eleni Pitsillou, Julia Liang, Andrew Hung, Tom C. Karagiannis
Life Sciences.2021; 265: 118809. CrossRef
Health benefits of dietary chronobiotics: beyond resynchronizing internal clocks
Jun-qing Huang, Muwen Lu, Chi-Tang Ho
Food & Function.2021; 12(14): 6136. CrossRef
Dietary Pterostilbene and Resveratrol Modulate the Gut Microbiota Influenced by Circadian Rhythm Dysregulation
Yen‐Chun Koh, Pei‐Sheng Lee, Yu‐Lun Kuo, Kalyanam Nagabhushanam, Chi‐Tang Ho, Min‐Hsiung Pan
Molecular Nutrition & Food Research.2021;[Epub] CrossRef
Systems Level Understanding of Circadian Integration with Cell Physiology
Andrew R. Morris, Daniel L. Stanton, Destino Roman, Andrew C. Liu
Journal of Molecular Biology.2020; 432(12): 3547. CrossRef
Quercetin, caffeic acid and resveratrol regulate circadian clock genes and aging-related genes in young and old human lung fibroblast cells
Yoshinori Okada, Mizue Okada
Molecular Biology Reports.2020; 47(2): 1021. CrossRef
Therapeutic effects of hydro-alcoholic leaf extract of Withania somnifera on age-induced changes in daily rhythms of Sirt1, Nrf2 and Rev-erbα in the SCN of male Wistar rats
Kowshik Kukkemane, Anita Jagota
Biogerontology.2020; 21(5): 593. CrossRef
Pharmacological basis and new insights of resveratrol action in the cardiovascular system
Chak Kwong Cheng, Jiang‐Yun Luo, Chi Wai Lau, Zhen‐Yu Chen, Xiao Yu Tian, Yu Huang
British Journal of Pharmacology.2020; 177(6): 1258. CrossRef
Effects of resveratrol on the levels of ATP, 5-HT and GAP-43 in the hippocampus of mice exposed to chronic unpredictable mild stress
Ji-Duo Shen, Yue-Wen Zhang, Bao-Ying Wang, Li Bai, Shuai-Fei Lu, Lei-Lei Zhu, Ming Bai, Yu-Cheng Li, Er-Ping Xu
Neuroscience Letters.2020; 735: 135232. CrossRef
Resveratrol and Vascular Function
Li, Xia, Hasselwander, Daiber
International Journal of Molecular Sciences.2019; 20(9): 2155. CrossRef
Sirt1 Promotes Osteogenic Differentiation and Increases Alveolar Bone Mass via Bmi1 Activation in Mice
Hua Wang, Zixuan Hu, Jun Wu, Yukun Mei, Qian Zhang, Hengwei Zhang, Dengshun Miao, Wen Sun
Journal of Bone and Mineral Research.2019; 34(6): 1169. CrossRef
RNA-Sequencing Analysis Reveals l-Theanine Regulating Transcriptional Rhythm Alteration in Vascular Smooth Muscle Cells Induced by Dexamethasone
Ruru Wang, Menchao Xiao, Yujing Zhang, Chi-Tang Ho, Xiaochun Wan, Daxiang Li, Zhongwen Xie
Journal of Agricultural and Food Chemistry.2019; 67(19): 5413. CrossRef
The effects of phytochemicals on circadian rhythm and related diseases
Tao Xu, Baiyi Lu
Critical Reviews in Food Science and Nutrition.2019; 59(6): 882. CrossRef
Effect of piceatannol on circadian Per2 expression in vitro and in vivo
Takayuki Yamamoto, Shiho Iwami, Shinya Aoyama, Hiroko Maruki-Uchida, Sadao Mori, Rina Hirooka, Kengo Takahashi, Minoru Morita, Shigenobu Shibata
Journal of Functional Foods.2019; 56: 49. CrossRef
AMPK/SIRT1/p38 MAPK signaling pathway regulates alcohol‑induced neurodegeneration by resveratrol
Xinyi Gu, Zhengxu Cai, Ming Cai, Kun Liu, Dan Liu, Qinsong Zhang, Jing Tan, Qiang Ma
Molecular Medicine Reports.2018;[Epub] CrossRef
Effects of fluctuating temperature and food availability on reproduction and lifespan
Tonia S. Schwartz, Phillip Pearson, John Dawson, David B. Allison, Julia M. Gohlke
Experimental Gerontology.2016; 86: 62. CrossRef
Multiple Integrated Complementary Healing Approaches: Energetics & Light for bone
Michael G. Gray, Brett R. Lackey, Evelyn F. Patrick, Sandra L. Gray, Susan G. Hurley
Medical Hypotheses.2016; 86: 18. CrossRef
Effects of BMAL1–SIRT1-positive cycle on estrogen synthesis in human ovarian granulosa cells: an implicative role of BMAL1 in PCOS
Jiaou Zhang, Jiansheng Liu, Kai Zhu, Yan Hong, Yun Sun, Xiaoming Zhao, Yanzhi Du, Zi-Jiang Chen
Endocrine.2016; 53(2): 574. CrossRef
Articles in 'Endocrinology and Metabolism' in 2014
Won-Young Lee
Endocrinology and Metabolism.2015; 30(1): 47. CrossRef

First
Prev
Page of 1
Next
Last

Search