Endocrinol Metab > Volume 20(5); 2005 > Article
Journal of Korean Endocrine Society 2005;20(5):476-487.
DOI: https://doi.org/10.3803/jkes.2005.20.5.476    Published online October 1, 2005.
Effects of alpha-Lipoic Acid on Bone Metabolism in Rats with Low Bone Mass.
Jung Min Koh, Hee Sook Lee, Duk Jae Kim, Ghi Su Kim
1Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Korea.
2Asan Institute for Life Science, Korea.
Abstract
BACKGROUND
Growing evidence has shown a biochemical link between increased oxidative stress and reduced bone density. In our previous study, alpha-lipoic acid (alpha-LA), a thiol antioxidant, suppressed both osteoclastogenesis and bone resorption, and also prevented TNF-alpha-induced apoptosis of osteoblast lineages. The effects of alpha-LA were investigated on bone metabolism in rats with a low bone mass. METHODS: An ovariectomy (OVX) or Talc injection (inflammation-mediated osteopenia, IMO) was performed in 12 week old female Sprague-Dawley rats. Diets containing either 0.3%, 0.5% or 1.0% alpha-LA were administered to the OVX rats for 16 weeks, and to the IMO rats for 21 days. The bone mineral densities (BMD) of the anterior-posterior lumbar spine and total femur were measured using dual-energy X-ray absorptiometry (Hologic QDR 4500-A), with small animal software. The plasma bone specific alkaline phosphatase activity (BSAP) and urinary free deoxypyridinoline concentration (DPD) were determined using enzyme immunoassay methods. RESULTS: The body weights were significantly decreased in the OVX rats on the diets containing 0.3 and 0.5% alpha-LA than in the OVX control. No significant differences in the BMD at either site were noted between rats administered the diets with or without alpha-LA. However, the administration of various doses of alpha-LA noticeably decreased the level of urinary DPD in both the OVX and IMO rats. High doses of alpha-LA (0.5% and/or 1.0%) also decreased the levels of plasma BSAP in both models. CONCLUSION: Although no increase in BMD was demonstrated by the administration of alpha-LA, these results suggest that alpha-LA suppresses the rates of bone turnover in rats with a low bone mass
Key Words: Oxidative stress, Antioxidants, alpha-Lipoic acid, Bone Density, Osteoporosis, Rats


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