Warning: fopen(/home/virtual/enm-kes/journal/upload/ip_log/ip_log_2024-03.txt): failed to open stream: Permission denied in /home/virtual/lib/view_data.php on line 88 Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 89 Implications of Mitochondrial Unfolded Protein Response and Mitokines: A Perspective on Fatty Liver Diseases
Skip Navigation
Skip to contents

Endocrinol Metab : Endocrinology and Metabolism

clarivate
OPEN ACCESS
SEARCH
Search

Articles

Page Path
HOME > Endocrinol Metab > Volume 34(1); 2019 > Article
Review Article
Implications of Mitochondrial Unfolded Protein Response and Mitokines: A Perspective on Fatty Liver Diseases
Hyon-Seung Yiorcid
Endocrinology and Metabolism 2019;34(1):39-46.
DOI: https://doi.org/10.3803/EnM.2019.34.1.39
Published online: March 21, 2019

Research Center for Endocrine and Metabolic Diseases, Chungnam National University College of Medicine, Daejeon, Korea.

Corresponding author: Hyon-Seung Yi. Research Center for Endocrine and Metabolic Diseases, Chungnam National University College of Medicine, 266 Munhwa-ro, Jung-gu, Daejeon 35015, Korea. Tel: +82-42-280-6994, Fax: +82-42-280-6990, jmpbooks@cnu.ac.kr
• Received: January 5, 2019   • Revised: January 16, 2019   • Accepted: January 24, 2019

Copyright © 2019 Korean Endocrine Society

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

  • 5,725 Views
  • 139 Download
  • 25 Web of Science
  • 25 Crossref
  • 22 Scopus
  • The signaling network of the mitochondrial unfolded protein response (UPRmt) and mitohormesis is a retrograde signaling pathway through which mitochondria-to-nucleus communication occurs in organisms. Recently, it has been shown that the UPRmt is closely associated with metabolic disorders and conditions involving insulin resistance, such as alcoholic and non-alcoholic fatty liver and fibrotic liver disease. Scientific efforts to understand the UPRmt and mitohormesis, as well as to establish the mitochondrial proteome, have established the importance of mitochondrial quality control in the development and progression of metabolic liver diseases, including non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH). In this review, we integrate and discuss the recent data from the literature on the UPRmt and mitohormesis in metabolic liver diseases, including NAFLD/NASH and fibrosis.
Metabolic liver diseases constitute a major health burden. Among them, fatty liver, characterized by the presence of large lipid vacuoles within the cytosol in hepatocytes, is a benign condition, but pathognomonic for non-alcoholic fatty liver disease (NAFLD) and alcoholic liver injury. Despite the globally widespread incidence and prevalence of NAFLD and non-alcoholic steatohepatitis (NASH), almost no pharmacotherapeutic options are available for these conditions. In approximately 10% of patients, NAFLD can progress in the long term to NASH, which can lead to fibrosis, cirrhosis, and hepatocellular carcinoma [1].
Liver parenchymal cells display a prominent eosinophilic cytoplasm upon hematoxylin-eosin staining, indicating that they have abundant mitochondria [2]. This means that mitochondrial dysfunction in hepatocytes results in cellular damage, which is linked to fatty liver diseases. For instance, chronic alcohol consumption alters mitochondrial oxidative phosphorylation in the liver by suppressing the synthesis of respiratory complex proteins [3], and hepatic mitochondria are structurally and functionally altered in NAFLD [4]. Thus, it is not surprising that mitochondrial dysfunction involving structural and molecular alterations leads to metabolic disturbances, potentially resulting in metabolic liver diseases, ranging from fatty liver to hepatocellular carcinoma.
Recently, one of the most remarkable scientific discoveries in mitochondrial biology has been the identification of the mitochondrial unfolded protein response (UPRmt), a retrograde mitochondria-to-nucleus signaling pathway induced by mitochondrial proteotoxic stress (Fig. 1). The UPRmt was first identified in the late 1990s, and is an area of increased scientific interest for research on aging-related degenerative diseases and metabolic disorders in organisms ranging from worms to mammals [5]. Regarding liver diseases, eliciting the UPRmt or mitohormetic factors not only inhibits the progression of NAFLD [6], but also ameliorates hepatic fibrosis and alcoholic liver injury in mice [7].
In this review, we summarize the current knowledge on the implications of mitochondrial function and protein homeostasis in the development of metabolic liver diseases, including NAFLD/NASH, alcoholic liver disease, and fibrosis. We also discuss mitochondrial proteostasis and mitohormesis as new therapeutic targets for the treatment of a broad spectrum of metabolic liver diseases.
Metabolic liver diseases are associated with functional alterations of mitochondrial oxidative phosphorylation [89]. For example, hepatic depletion of mitochondrial flavoprotein apoptosis inducing factor induced deficient oxidative phosphorylation, but resulted in the improvement of hepatic insulin resistance in mice [10]. In contrast, alcohol-mediated mitochondrial DNA (mtDNA) damage deteriorated cellular energy metabolism via enhanced formation of reactive oxygen species (ROS) in mice [11]. Moreover, in another mouse study, carbon tetrachloride (CCl4) decreased mitochondrial respiratory chain complex IV activity and reduced hepatic mtDNA [12]. Furthermore, the ROS produced by cytochrome P450 2E1-mediated CCl4 metabolism has been found to bind to mtDNA and promote lipid peroxidation, leading to mtDNA degradation [12]. Thus, ROS-mediated hepatocellular oxidative damage is implicated in the progression of NAFLD and fibrotic liver diseases.
Recent investigations in worms and mammals have demonstrated that genetic inhibition of mitochondrial oxidative phosphorylation activated the UPRmt, leading to an increased lifespan and improved metabolic phenotypes [13]. This suggests that modest mitochondrial inhibition paradoxically improves the metabolic phenotype and lifespan across species. This phenomenon, which is defined as mitochondrial homeostasis, or mitohormesis, involves the UPRmt and plays a prominent role in aging and degenerative diseases. Many scientists are trying to establish the role of the UPRmt in mammalian cells and systems, and to characterize its possible role in mitohormesis [5]. The mitochondrial chaperones and proteases predicted to be involved in the regulation of mitochondrial protein homeostasis may play a pivotal role in the modulation of fatty liver diseases in mammalian systems [5]. While elucidation of the UPRmt has contributed to a greater understanding of the role of mitochondria in stress adaptation and lifespan regulation, important questions remain unanswered about the role of the UPRmt in the development of fatty liver and fibrotic liver diseases.
Mitochondrial chaperones
Mitochondrial chaperones and proteases play important roles in normal cellular function and survival, which are required for maintaining organismal homeostasis. Emerging studies have shown that the biological effects of UPRmt activation in conditions of increased mitochondrial proteotoxic stress are mediated by both cell autonomous and cell non-autonomous factors [14]. In the liver, heat shock protein 60 (HSP60) is involved in NAFLD/NASH, chronic hepatitis, and liver cancer [15]. Hsp60 works as a major defense system against proteotoxic cellular damage during alcoholic liver injury [16]. Heat shock protein family D member 1 (Hspd1) was also found to be an essential factor in regulating the inflammatory response and cell proliferation during tissue regeneration in zebrafish [17]. Deficiency of Hspd1 resulted in acute hepatic injury and cholangiocellular tumorigenesis via hepatic mitochondrial dysfunction [18]. In contrast, Hsp90 increased lipid accumulation in the liver by modulating peroxisome proliferator-activated receptor γ (PPARγ) activity in a mouse model of NAFLD [19]. Additionally, a recent proteomic analysis found that both Hsp90 isoforms were elevated in patients with NAFLD, suggesting a link between NAFLD and Hsp90 in humans [20]. Deletion of prohibitin 1 in the liver promotes liver injury, oxidative stress, inflammation, and fibrosis with development of liver cancer [2122]. HSP72 is also involved in chronic liver diseases. HSP72 mRNA expression in skeletal muscle was found to be negatively correlated with the insulin-stimulated glucose disposal rate in patients with type 2 diabetes in a hyperinsulinemic-euglycemic clamp study [23]. Patients with NASH or chronic hepatitis C virus infection showed an increase in HSP72 levels [24]. Mice with hepatocyte-specific Hsp72 overexpression were protected from acute or chronic liver injury induced by a single injection of acetaminophen, a methionine-/choline-deficient diet, or a 3,5-diethoxycarbonyl-1,4-dihydrocollidine-supplemented diet [24].
Mitochondrial proteases
In addition to the mitochondrial chaperone system, many kinds of proteases are also evolutionarily conserved for mitochondrial quality control. Similar to chaperones, mitochondrial proteases are predicted to be involved in the regulation of mitochondrial protein homeostasis, thereby playing an essential role in the modulation of metabolic diseases across species. The inner mitochondrial membrane protease OMA1 zinc metallopeptidase (OMA1) plays an essential role in the proteolytic inactivation of the GTPase optic atrophy protein 1 (OPA1), which regulates mitochondrial quality control. A remarkable level of steatosis was found in the liver of Oma1-deficient mice on a normal chow diet, and was further significantly elevated under a high-fat diet [25]. OPA1 is required for fusion of the outer mitochondrial membrane and inner mitochondrial membrane in mammals [26]. Acute Opa1 deletion in skeletal muscle was found to induce hepatic steatosis and high levels of inflammatory cytokines in mice [27]. Mitofusin-2-mediated proteolytic inactivation of Opa1 was found to be associated with mitochondria-endoplasmic reticulum contact assembly in the postprandial liver [28]. LON protease (also known as LONP1) is an ATP-dependent serine protease that is essential for mitochondrial protein homeostasis within the mitochondrial matrix. Reduction of LONP1 by small interfering RNA increased intracellular ROS production in liver cells [29]. Downregulation of mitochondrial LON protease also promoted hepatic glucose production by inducing glucose-6-phosphatase and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) in human liver SK-HEP-1 cells [29]. Moreover, deficiency of LONP1 also resulted in gluconeogenesis by induction of glucose-6-phosphatase and PGC-1α in human liver SK-HEP-1 cells [29]. Furthermore, ATP-stimulated LONP1 activity was remarkably reduced in the hepatic mitochondrial matrix with aging in rats [30]. Caseinolytic peptidase P (ClpP), another matrix protease, plays a crucial role in the initiation of the mammalian UPRmt. ClpP−/− mice showed reduced adiposity and enhanced insulin-stimulated Akt phosphorylation in the liver [31]. ClpP−/− mice were protected against diet-induced obesity, insulin resistance, and fatty liver under a high-fat diet [31]. This paradoxical beneficial effect of ClpP deficiency is due to mitochondrial matrix proteostasis through upregulation of mitochondrial chaperones and proteases.
Fibroblast growth factor 21
Remarkable studies in worms have established a class of secretory proteins defined as mitochondrial cytokines or mitokines [32]. Mitokine responses in mammalian system can be regarded as critical cell non-autonomous factors in disease development and progression. Individual mitokines can be used as disease markers, and these mitohormetic factors may serve as potential therapeutics for metabolic diseases including NAFLD/NASH and insulin resistance [5].
Fibroblast growth factor 21 (FGF21) was the first mitokine reported in mammalian systems. Under physiologic perturbations such as fasting, the liver is the most important organ that secretes FGF21 into the circulation, suggesting that circulating FGF21 derives largely from the liver [33]. However, FGF21 expression is markedly induced in muscle myopathies induced by mitochondrial stress [7]. Specifically, FGF21 stimulates β-oxidation of fatty acids, as well as the production of hepatic ketone bodies, and suppresses lipogenesis in the liver [34].
Impaired mitochondrial function resulted in activating transcription factor 4 (ATF4)-dependent FGF21 induction, leading to protection from diet-induced obesity and hepatic steatosis in mice [35]. In addition, FGF21 inhibited hepatic steatosis and enhanced hepatic insulin sensitivity by lowering hepatic gluconeogenesis and increasing hepatic glycogen content, thereby leading to improvements in systemic glucose intolerance and insulin resistance in mice with diet-induced obesity [3637]. Moreover, FGF21 reduced low density lipoprotein cholesterol and increased high density lipoprotein cholesterol in the serum of non-human primates [38]. FGF21 has been found to ameliorate alcohol-induced fatty liver by activating the AMP-activated protein kinase-sirtuin 1 pathway, and by attenuating intracellular ROS production [39]. Furthermore, administration of recombinant human FGF21 decreased chronic alcohol-induced hepatic steatosis and inflammation through increased fatty acid β-oxidation and decreased lipogenesis in mice [40]. FGF21-deficient mice were found to have worsened steatosis, inflammation, and fibrosis under a methionine- and choline-deficient diet for 8 to 16 weeks [41]. Treating mice with recombinant FGF21 also decreased hepatic fibrosis and prevented induction of fibrotic mediators in the liver by regulating the transforming growth factor β (TGF-β)/Smad2/3 and nuclear factor κB (NF-κB) signaling pathways [42].
The receptors for FGF21 consists of FGF receptor (FGFR) and βKlotho, which activate FGF21 signal transduction [43]. Among FGFRs widely distributed in the body, FGFR1c is a key receptor for FGF21, and other receptors mediating FGF21 signaling include FGFR2 and FGFR4 [44]. Tissue-specific expression of βKlotho may determine the biological functions of FGF21 because FGFRs are ubiquitously expressed in the many kinds of tissues [45].
Growth differentiation factor 15
Growth differentiation factor 15 (GDF15), a member of the TGF-β superfamily, has anti-inflammatory activities in mammalian systems [46]. Recently, dysfunctional mitochondrial oxidative phosphorylation has been found to promote the induction of GDF15, a mitokine regulating systemic energy metabolism in mice [47]. C/EBP-homologous protein (CHOP), which is part of the unfolded protein response signaling pathway in the mitochondria and endoplasmic reticulum, directly binds to the promoter of GDF15 and activates its transcription [4748]. GDF15 is induced by the cellular adaptive response to tissue injury or exposure to carcinogens in a number of solid organs [49]. Additionally, GDF15 as a serum biomarker predicts liver diseases including NAFLD, NASH, and advanced liver fibrosis in humans [50]. Elevated levels of GDF15 are also closely associated with increased risk of chronic liver and cardiovascular diseases [5].
Treatment with recombinant GDF15 reduced fat accumulation in the liver and attenuated NAFLD progression by enhancing hepatic fatty acid β-oxidation in mice fed a high-fat diet [4751]. In contrast, abrogation of hepatic Gdf15 decreased fatty acid β-oxidation and ketone production in fasting mice [52]. Knockdown of hepatic Gdf15 also reduced ketogenesis in streptozotocin-induced diabetic mice, as well as mice fed a ketogenic diet [52]. GDF15 deficiency exacerbated hepatic fat deposition and the induction of pro-inflammatory cytokines in mice fed a liquid ethanol diet [7]. Moreover, GDF15 deficiency promoted liver inflammation and the expression of fibrotic mediators in a CCl4-induced mouse model [7]. Furthermore, GDF15 inhibited the growth and activity of fibroblasts via the modulating TGF-β-Smad signaling pathway in fibroblasts [53].
Recently, several emerging studies have identified that GDNF family receptor α-like (GFRAL) in the hindbrain is the receptor for GDF15 and a therapeutic target for the treatment of obesity and metabolic diseases [545556]. However, the receptors for GDF15 in peripheral tissues have not yet been identified, although GDF15 has been shown to play a variety of roles in peripheral organs. Thus, further studies are required to understand the peripheral roles of GDF15 in metabolic organs such as the liver, adipose tissue, and skeletal muscle.
Modest or transient mitochondrial stress induces UPRmt and mitohormesis in mammalian and worms. This response may provide protection or amelioration to the host in both a cell autonomous and cell non-autonomous manner. Thus, identification of UPRmt regulators may serve to restore or prevent metabolic deterioration and the development of fatty liver diseases.
Recently, administration of nicotinamide riboside, a precursor of NAD+ biosynthesis, has been shown to protect against hepatic fat accumulation in mice fed a high-fat and high-sucrose diet by sirtuin 1 and sirtuin 3-mediated UPRmt, triggering a mitohormetic pathway that enhanced hepatic β-oxidation and mitochondrial complex activity [657]. In addition, a recent study revealed that activating transcription factor 5 is required to maintain mammalian UPRmt during mitochondrial stress, which is essential for organelle recovery [58]. Therefore, further studies to discover the upstream regulators of UPRmt and mitohormesis will provide clues for developing a new class of treatments for fatty liver diseases in humans.
This review has highlighted the UPRmt and two kinds of mitokines in mammalian systems, which play a protective role in fatty liver and fibrotic liver diseases. However, some important questions remain regarding the mammalian UPRmt and mitohormesis in the development and progression of NAFLD/NASH, alcoholic liver disease, and fibrosis. It would be interesting to determine how UPRmt and mitokines affect systemic energy metabolism, leading to the attenuation of fatty liver and metabolic liver disease. Additionally, we should identify the upstream regulators of UPRmt and mitohormesis in the liver, which might lead to the discovery of drugs for metabolic liver diseases including NAFLD/NASH. Furthermore, several publications have suggested that receptors for mitokines such as GDF15 may be present in peripheral metabolic organs [74750]. Thus, further studies regarding mitokine receptors could provide clues about inter-organ communication by mitohormesis in metabolic liver diseases.
Acknowledgements
This work was supported by the Korean Endocrine Society of New Faculty Research Award 2018 and Chungnam National University Hospital Research Fund, 2018. Hyon-Seung Yi was also supported by the Basic Science Research Program, National Research Foundation, Ministry of Science and ICT, Future Planning, Korea (NRF-2018R1C1B6004439).

CONFLICTS OF INTEREST: No potential conflict of interest relevant to this article was reported.

  • 1. Begriche K, Massart J, Robin MA, Bonnet F, Fromenty B. Mitochondrial adaptations and dysfunctions in nonalcoholic fatty liver disease. Hepatology 2013;58:1497–1507. ArticlePubMed
  • 2. Nassir F, Ibdah JA. Role of mitochondria in alcoholic liver disease. World J Gastroenterol 2014;20:2136–2142. ArticlePubMedPMC
  • 3. Wei YH, Chen YS, Lee JF, Huang JY, Lee CH. Effect of ethanol intake on rat liver mitochondrial respiration and oxidative phosphorylation. Proc Natl Sci Counc Repub China B 1990;14:61–68. PubMed
  • 4. Einer C, Hohenester S, Wimmer R, Wottke L, Artmann R, Schulz S, et al. Mitochondrial adaptation in steatotic mice. Mitochondrion 2018;40:1–12. ArticlePubMed
  • 5. Yi HS, Chang JY, Shong M. The mitochondrial unfolded protein response and mitohormesis: a perspective on metabolic diseases. J Mol Endocrinol 2018;61:R91–R105. ArticlePubMedPMC
  • 6. Gariani K, Menzies KJ, Ryu D, Wegner CJ, Wang X, Ropelle ER, et al. Eliciting the mitochondrial unfolded protein response by nicotinamide adenine dinucleotide repletion reverses fatty liver disease in mice. Hepatology 2016;63:1190–1204. ArticlePubMed
  • 7. Chung HK, Kim JT, Kim HW, Kwon M, Kim SY, Shong M, et al. GDF15 deficiency exacerbates chronic alcohol- and carbon tetrachloride-induced liver injury. Sci Rep 2017;7:17238ArticlePubMedPMCPDF
  • 8. Ciapaite J, Bakker SJ, Van Eikenhorst G, Wagner MJ, Teerlink T, Schalkwijk CG, et al. Functioning of oxidative phosphorylation in liver mitochondria of high-fat diet fed rats. Biochim Biophys Acta 2007;1772:307–316. ArticlePubMed
  • 9. Garcia-Ruiz I, Solis-Munoz P, Fernandez-Moreira D, Grau M, Colina F, Munoz-Yague T, et al. High-fat diet decreases activity of the oxidative phosphorylation complexes and causes nonalcoholic steatohepatitis in mice. Dis Model Mech 2014;7:1287–1296. ArticlePubMedPMC
  • 10. Pospisilik JA, Knauf C, Joza N, Benit P, Orthofer M, Cani PD, et al. Targeted deletion of AIF decreases mitochondrial oxidative phosphorylation and protects from obesity and diabetes. Cell 2007;131:476–491. ArticlePubMed
  • 11. Mansouri A, Gaou I, De Kerguenec C, Amsellem S, Haouzi D, Berson A, et al. An alcoholic binge causes massive degradation of hepatic mitochondrial DNA in mice. Gastroenterology 1999;117:181–190. ArticlePubMed
  • 12. Knockaert L, Berson A, Ribault C, Prost PE, Fautrel A, Pajaud J, et al. Carbon tetrachloride-mediated lipid peroxidation induces early mitochondrial alterations in mouse liver. Lab Invest 2012;92:396–410. ArticlePubMedPDF
  • 13. Tian Y, Garcia G, Bian Q, Steffen KK, Joe L, Wolff S, et al. Mitochondrial stress induces chromatin reorganization to promote longevity and UPR(mt). Cell 2016;165:1197–1208. ArticlePubMedPMC
  • 14. Aldridge JE, Horibe T, Hoogenraad NJ. Discovery of genes activated by the mitochondrial unfolded protein response (mtUPR) and cognate promoter elements. PLoS One 2007;2:e874. ArticlePubMedPMC
  • 15. Cappello F, Marino Gammazza A, Palumbo Piccionello A, Campanella C, Pace A, Conway de Macario E, et al. Hsp60 chaperonopathies and chaperonotherapy: targets and agents. Expert Opin Ther Targets 2014;18:185–208. ArticlePubMed
  • 16. Rakonczay Z Jr, Boros I, Jarmay K, Hegyi P, Lonovics J, Takacs T. Ethanol administration generates oxidative stress in the pancreas and liver, but fails to induce heat-shock proteins in rats. J Gastroenterol Hepatol 2003;18:858–867. ArticlePubMed
  • 17. Pei W, Tanaka K, Huang SC, Xu L, Liu B, Sinclair J, et al. Extracellular HSP60 triggers tissue regeneration and wound healing by regulating inflammation and cell proliferation. NPJ Regen Med 2016;1:16013ArticlePubMedPMCPDF
  • 18. Yuan D, Huang S, Berger E, Liu L, Gross N, Heinzmann F, et al. Kupffer cell-derived TNF triggers cholangiocellular tumorigenesis through JNK due to chronic mitochondrial dysfunction and ROS. Cancer Cell 2017;31:771–789. ArticlePubMedPMC
  • 19. Wheeler MC, Gekakis N. Hsp90 modulates PPARγ activity in a mouse model of nonalcoholic fatty liver disease. J Lipid Res 2014;55:1702–1710. ArticlePubMedPMC
  • 20. Rodriguez-Suarez E, Duce AM, Caballeria J, Martinez Arrieta F, Fernandez E, Gomara C, et al. Non-alcoholic fatty liver disease proteomics. Proteomics Clin Appl 2010;4:362–371. ArticlePubMedPMC
  • 21. Ko KS, Tomasi ML, Iglesias-Ara A, French BA, French SW, Ramani K, et al. Liver-specific deletion of prohibitin 1 results in spontaneous liver injury, fibrosis, and hepatocellular carcinoma in mice. Hepatology 2010;52:2096–2108. ArticlePubMedPMC
  • 22. Fan W, Yang H, Liu T, Wang J, Li TW, Mavila N, et al. Prohibitin 1 suppresses liver cancer tumorigenesis in mice and human hepatocellular and cholangiocarcinoma cells. Hepatology 2017;65:1249–1266. ArticlePubMedPMC
  • 23. Bruce CR, Carey AL, Hawley JA, Febbraio MA. Intramuscular heat shock protein 72 and heme oxygenase-1 mRNA are reduced in patients with type 2 diabetes: evidence that insulin resistance is associated with a disturbed antioxidant defense mechanism. Diabetes 2003;52:2338–2345. ArticlePubMed
  • 24. Levada K, Guldiken N, Zhang X, Vella G, Mo FR, James LP, et al. Hsp72 protects against liver injury via attenuation of hepatocellular death, oxidative stress, and JNK signaling. J Hepatol 2018;68:996–1005. ArticlePubMedPMC
  • 25. Quiros PM, Ramsay AJ, Sala D, Fernandez-Vizarra E, Rodriguez F, Peinado JR, et al. Loss of mitochondrial protease OMA1 alters processing of the GTPase OPA1 and causes obesity and defective thermogenesis in mice. EMBO J 2012;31:2117–2133. ArticlePubMedPMC
  • 26. Romanello V, Sandri M. Mitochondrial quality control and muscle mass maintenance. Front Physiol 2016;6:422ArticlePubMedPMC
  • 27. Tezze C, Romanello V, Desbats MA, Fadini GP, Albiero M, Favaro G, et al. Age-associated loss of OPA1 in muscle impacts muscle mass, metabolic homeostasis, systemic inflammation, and epithelial senescence. Cell Metab 2017;25:1374–1389. ArticlePubMedPMC
  • 28. Sood A, Jeyaraju DV, Prudent J, Caron A, Lemieux P, McBride HM, et al. A mitofusin-2-dependent inactivating cleavage of Opa1 links changes in mitochondria cristae and ER contacts in the postprandial liver. Proc Natl Acad Sci U S A 2014;111:16017–16022. ArticlePubMedPMC
  • 29. Lee HJ, Chung K, Lee H, Lee K, Lim JH, Song J. Downregulation of mitochondrial lon protease impairs mitochondrial function and causes hepatic insulin resistance in human liver SK-HEP-1 cells. Diabetologia 2011;54:1437–1446. ArticlePubMedPDF
  • 30. Bakala H, Delaval E, Hamelin M, Bismuth J, Borot-Laloi C, Corman B, et al. Changes in rat liver mitochondria with aging. Lon protease-like reactivity and N(epsilon)-carboxymethyllysine accumulation in the matrix. Eur J Biochem 2003;270:2295–2302. ArticlePubMed
  • 31. Bhaskaran S, Pharaoh G, Ranjit R, Murphy A, Matsuzaki S, Nair BC, et al. Loss of mitochondrial protease ClpP protects mice from diet-induced obesity and insulin resistance. EMBO Rep 2018;19:e45009. ArticlePubMedPMC
  • 32. Durieux J, Wolff S, Dillin A. The cell-non-autonomous nature of electron transport chain-mediated longevity. Cell 2011;144:79–91. ArticlePubMedPMC
  • 33. Markan KR, Naber MC, Ameka MK, Anderegg MD, Mangelsdorf DJ, Kliewer SA, et al. Circulating FGF21 is liver derived and enhances glucose uptake during refeeding and overfeeding. Diabetes 2014;63:4057–4063. ArticlePubMedPMC
  • 34. Badman MK, Pissios P, Kennedy AR, Koukos G, Flier JS, Maratos-Flier E. Hepatic fibroblast growth factor 21 is regulated by PPARalpha and is a key mediator of hepatic lipid metabolism in ketotic states. Cell Metab 2007;5:426–437. ArticlePubMed
  • 35. Kim KH, Jeong YT, Oh H, Kim SH, Cho JM, Kim YN, et al. Autophagy deficiency leads to protection from obesity and insulin resistance by inducing Fgf21 as a mitokine. Nat Med 2013;19:83–92. ArticlePubMedPDF
  • 36. Berglund ED, Li CY, Bina HA, Lynes SE, Michael MD, Shanafelt AB, et al. Fibroblast growth factor 21 controls glycemia via regulation of hepatic glucose flux and insulin sensitivity. Endocrinology 2009;150:4084–4093. ArticlePubMedPMCPDF
  • 37. Xu J, Lloyd DJ, Hale C, Stanislaus S, Chen M, Sivits G, et al. Fibroblast growth factor 21 reverses hepatic steatosis, increases energy expenditure, and improves insulin sensitivity in diet-induced obese mice. Diabetes 2009;58:250–259. ArticlePubMedPMC
  • 38. Kharitonenkov A, Wroblewski VJ, Koester A, Chen YF, Clutinger CK, Tigno XT, et al. The metabolic state of diabetic monkeys is regulated by fibroblast growth factor-21. Endocrinology 2007;148:774–781. ArticlePubMedPDF
  • 39. Zhu S, Ma L, Wu Y, Ye X, Zhang T, Zhang Q, et al. FGF21 treatment ameliorates alcoholic fatty liver through activation of AMPK-SIRT1 pathway. Acta Biochim Biophys Sin (Shanghai) 2014;46:1041–1048. ArticlePubMedPDF
  • 40. Liu Y, Zhao C, Xiao J, Liu L, Zhang M, Wang C, et al. Fibroblast growth factor 21 deficiency exacerbates chronic alcohol-induced hepatic steatosis and injury. Sci Rep 2016;6:31026ArticlePubMedPMCPDF
  • 41. Fisher FM, Chui PC, Nasser IA, Popov Y, Cunniff JC, Lundasen T, et al. Fibroblast growth factor 21 limits lipotoxicity by promoting hepatic fatty acid activation in mice on methionine and choline-deficient diets. Gastroenterology 2014;147:1073–1083. ArticlePubMedPMC
  • 42. Xu P, Zhang Y, Liu Y, Yuan Q, Song L, Liu M, et al. Fibroblast growth factor 21 attenuates hepatic fibrogenesis through TGF-β/smad2/3 and NF-κB signaling pathways. Toxicol Appl Pharmacol 2016;290:43–53. ArticlePubMed
  • 43. Kharitonenkov A, Dunbar JD, Bina HA, Bright S, Moyers JS, Zhang C, et al. FGF-21/FGF-21 receptor interaction and activation is determined by betaKlotho. J Cell Physiol 2008;215:1–7. ArticlePubMed
  • 44. Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, et al. BetaKlotho is required for metabolic activity of fibroblast growth factor 21. Proc Natl Acad Sci U S A 2007;104:7432–7437. ArticlePubMedPMC
  • 45. Fon Tacer K, Bookout AL, Ding X, Kurosu H, John GB, Wang L, et al. Research resource: Comprehensive expression atlas of the fibroblast growth factor system in adult Mouse. Mol Endocrinol 2010;24:2050–2064. ArticlePubMedPMCPDF
  • 46. Breit SN, Johnen H, Cook AD, Tsai VW, Mohammad MG, Kuffner T, et al. The TGF-β superfamily cytokine, MIC-1/GDF15: a pleotrophic cytokine with roles in inflammation, cancer and metabolism. Growth Factors 2011;29:187–195. ArticlePubMed
  • 47. Chung HK, Ryu D, Kim KS, Chang JY, Kim YK, Yi HS, et al. Growth differentiation factor 15 is a myomitokine governing systemic energy homeostasis. J Cell Biol 2017;216:149–165. ArticlePubMedPMCPDF
  • 48. Li D, Zhang H, Zhong Y. Hepatic GDF15 is regulated by CHOP of the unfolded protein response and alleviates NAFLD progression in obese mice. Biochem Biophys Res Commun 2018;498:388–394. ArticlePubMed
  • 49. Zimmers TA, Jin X, Hsiao EC, Perez EA, Pierce RH, Chavin KD, et al. Growth differentiation factor-15: induction in liver injury through p53 and tumor necrosis factor-independent mechanisms. J Surg Res 2006;130:45–51. ArticlePubMed
  • 50. Koo BK, Um SH, Seo DS, Joo SK, Bae JM, Park JH, et al. Growth differentiation factor 15 predicts advanced fibrosis in biopsy-proven non-alcoholic fatty liver disease. Liver Int 2018;38:695–705. ArticlePubMed
  • 51. Kim KH, Kim SH, Han DH, Jo YS, Lee YH, Lee MS. Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice. Sci Rep 2018;8:6789ArticlePubMedPMCPDF
  • 52. Zhang M, Sun W, Qian J, Tang Y. Fasting exacerbates hepatic growth differentiation factor 15 to promote fatty acid β-oxidation and ketogenesis via activating XBP1 signaling in liver. Redox Biol 2018;16:87–96. ArticlePubMedPMC
  • 53. Kim YI, Shin HW, Chun YS, Cho CH, Koh J, Chung DH, et al. Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis. Cell Death Dis 2018;9:506ArticlePubMedPMCPDF
  • 54. Emmerson PJ, Wang F, Du Y, Liu Q, Pickard RT, Gonciarz MD, et al. The metabolic effects of GDF15 are mediated by the orphan receptor GFRAL. Nat Med 2017;23:1215–1219. ArticlePubMedPDF
  • 55. Mullican SE, Lin-Schmidt X, Chin CN, Chavez JA, Furman JL, Armstrong AA, et al. GFRAL is the receptor for GDF15 and the ligand promotes weight loss in mice and nonhuman primates. Nat Med 2017;23:1150–1157. ArticlePubMedPDF
  • 56. Yang L, Chang CC, Sun Z, Madsen D, Zhu H, Padkjaer SB, et al. GFRAL is the receptor for GDF15 and is required for the anti-obesity effects of the ligand. Nat Med 2017;23:1158–1166. ArticlePubMedPDF
  • 57. Gariani K, Ryu D, Menzies KJ, Yi HS, Stein S, Zhang H, et al. Inhibiting poly ADP-ribosylation increases fatty acid oxidation and protects against fatty liver disease. J Hepatol 2017;66:132–141. ArticlePubMed
  • 58. Fiorese CJ, Schulz AM, Lin YF, Rosin N, Pellegrino MW, Haynes CM. The transcription factor ATF5 mediates a mammalian mitochondrial UPR. Curr Biol 2016;26:2037–2043. ArticlePubMedPMC
Fig. 1

Scheme depicting the mitochondrial unfolded protein response (UPRmt) and mitokines. As a cell autonomous response, damaged mitochondria within a cell communicate to the nucleus via retrograde signaling. In contrast, mitokines (as a cell non-autonomous factor) are induced during the UPRmt and are secreted from cells affected by mitochondrial stress. OXPHOS, oxidative phosphorylation; FGF21, fibroblast growth factor 21; GDF15, growth differentiation factor 15; Hspd1, heat shock protein family D member 1; ClpP, caseinolytic peptidase P; Lonp1, LON protease 1.

enm-34-39-g001.jpg

Figure & Data

References

    Citations

    Citations to this article as recorded by  
    • Aberrant mitochondrial aggregation of TDP-43 activated mitochondrial unfolded protein response and contributed to recovery of acetaminophen induced acute liver injury
      Zhaoxiong Liu, Yalong Qiang, Shulin Shan, Shuai Wang, Zhidan Liu, Yiyu Yang, Zhengcheng Huang, Mingxue Song, Xiulan Zhao, Fuyong Song
      Toxicology Research.2024;[Epub]     CrossRef
    • Autophagy and the unfolded protein response shape the non-alcoholic fatty liver landscape: decoding the labyrinth
      Zahra Dashti, Zeynab Yousefi, Pouria Kiani, Motahareh Taghizadeh, Mohammad Hasan Maleki, Mohammad Borji, Omid Vakili, Sayed Mohammad Shafiee
      Metabolism.2024; : 155811.     CrossRef
    • Recent progress in understanding mitokines as diagnostic and therapeutic targets in hepatocellular carcinoma
      Jiang Wang, Lan-Zhu Luo, Dao-Miao Liang, Chao Guo, Zhi-Hong Huang, Xiao-Hong Jian, Jie Wen
      World Journal of Clinical Cases.2023; 11(23): 5416.     CrossRef
    • From Non-Alcoholic Fatty Liver to Hepatocellular Carcinoma: A Story of (Mal)Adapted Mitochondria
      Ricardo Amorim, Carina C. Magalhães, Fernanda Borges, Paulo J. Oliveira, José Teixeira
      Biology.2023; 12(4): 595.     CrossRef
    • Natural Drugs: A New Direction for the Prevention and Treatment of Diabetes
      Peishan Wu, Xiaolei Wang
      Molecules.2023; 28(14): 5525.     CrossRef
    • Mitochondrial Quality Control via Mitochondrial Unfolded Protein Response (mtUPR) in Ageing and Neurodegenerative Diseases
      Paula Cilleros-Holgado, David Gómez-Fernández, Rocío Piñero-Pérez, Jose Manuel Romero-Domínguez, Diana Reche-López, Alejandra López-Cabrera, Mónica Álvarez-Córdoba, Manuel Munuera-Cabeza, Marta Talaverón-Rey, Alejandra Suárez-Carrillo, Ana Romero-González
      Biomolecules.2023; 13(12): 1789.     CrossRef
    • Heat Shock Protein 60 Restricts Release of Mitochondrial dsRNA to Suppress Hepatic Inflammation and Ameliorate Non-Alcoholic Fatty Liver Disease in Mice
      Ying-Hsien Huang, Feng-Sheng Wang, Pei-Wen Wang, Hung-Yu Lin, Sheng-Dean Luo, Ya-Ling Yang
      International Journal of Molecular Sciences.2022; 23(1): 577.     CrossRef
    • UPRmt activation improves pathological alterations in cellular models of mitochondrial diseases
      Juan M. Suárez-Rivero, Carmen J. Pastor-Maldonado, Suleva Povea-Cabello, Mónica Álvarez-Córdoba, Irene Villalón-García, Marta Talaverón-Rey, Alejandra Suárez-Carrillo, Manuel Munuera-Cabeza, Diana Reche-López, Paula Cilleros-Holgado, Rocío Piñero-Perez, J
      Orphanet Journal of Rare Diseases.2022;[Epub]     CrossRef
    • Umbilical Cord-Mesenchymal Stem Cell-Conditioned Medium Improves Insulin Resistance in C2C12 Cell
      Kyung-Soo Kim, Yeon Kyung Choi, Mi Jin Kim, Jung Wook Hwang, Kyunghoon Min, Sang Youn Jung, Soo-Kyung Kim, Yong-Soo Choi, Yong-Wook Cho
      Diabetes & Metabolism Journal.2021; 45(2): 260.     CrossRef
    • Mitochondrial unfolded protein response: An emerging pathway in human diseases
      Li Zhu, Qionglin Zhou, Lu He, Linxi Chen
      Free Radical Biology and Medicine.2021; 163: 125.     CrossRef
    • Mitochondrial dynamics and nonalcoholic fatty liver disease (NAFLD): new perspectives for a fairy-tale ending?
      Miriam Longo, Marica Meroni, Erika Paolini, Chiara Macchi, Paola Dongiovanni
      Metabolism.2021; 117: 154708.     CrossRef
    • Mitochondrial unfolded protein response: A novel pathway in metabolism and immunity
      Li Zhu, Xuling Luo, Nian Fu, Linxi Chen
      Pharmacological Research.2021; 168: 105603.     CrossRef
    • Mitochondrial Metabolic Signatures in Hepatocellular Carcinoma
      Ho-Yeop Lee, Ha Thi Nga, Jingwen Tian, Hyon-Seung Yi
      Cells.2021; 10(8): 1901.     CrossRef
    • Cnm1 mediates nucleus–mitochondria contact site formation in response to phospholipid levels
      Michal Eisenberg-Bord, Naama Zung, Javier Collado, Layla Drwesh, Emma J. Fenech, Amir Fadel, Nili Dezorella, Yury S. Bykov, Doron Rapaport, Ruben Fernandez-Busnadiego, Maya Schuldiner
      Journal of Cell Biology.2021;[Epub]     CrossRef
    • The Role of Carnitine Orotate Complex in Fatty Liver
      Hyon-Seung Yi
      Diabetes & Metabolism Journal.2021; 45(6): 866.     CrossRef
    • Serum GDF15 Level Is Independent of Sarcopenia in Older Asian Adults
      Ha Thi Nga, Il-Young Jang, Da Ae Kim, So Jeong Park, Jin Young Lee, Seungjoo Lee, Jeoung Hee Kim, Eunju Lee, Jin Hoon Park, Young-Ho Lee, Hyon-Seung Yi, Beom-Jun Kim
      Gerontology.2021; 67(5): 525.     CrossRef
    • Exogenous Therapeutics of Microrna-29a Attenuates Development of Hepatic Fibrosis in Cholestatic Animal Model through Regulation of Phosphoinositide 3-Kinase p85 Alpha
      Ya-Ling Yang, Feng-Sheng Wang, Hung-Yu Lin, Ying-Hsien Huang
      International Journal of Molecular Sciences.2020; 21(10): 3636.     CrossRef
    • High-altitude chronic hypoxia ameliorates obesity-induced non-alcoholic fatty liver disease in mice by regulating mitochondrial and AMPK signaling
      Kang Song, Yifan Zhang, Qin Ga, Zhenzhong Bai, Ri-Li Ge
      Life Sciences.2020; 252: 117633.     CrossRef
    • NRF-2 and nonalcoholic fatty liver disease
      Arturo Solano-Urrusquieta, José A. Morales-González, Graciela E. Castro-Narro, Eira Cerda-Reyes, Perla D. Flores-Rangel, Raul Fierros-Oceguera
      Annals of Hepatology.2020; 19(5): 458.     CrossRef
    • Mitochondrial Quality Control in the Heart: New Drug Targets for Cardiovascular Disease
      Chang-Myung Oh, Dongryeol Ryu, Sungsoo Cho, Yangsoo Jang
      Korean Circulation Journal.2020; 50(5): 395.     CrossRef
    • Growth differentiation factor 15 protects against the aging‐mediated systemic inflammatory response in humans and mice
      Ji Sun Moon, Ludger J. E. Goeminne, Jung Tae Kim, Jing Wen Tian, Seok‐Hwan Kim, Ha Thi Nga, Seul Gi Kang, Baeki E. Kang, Jin‐Seok Byun, Young‐Sun Lee, Jae‐Han Jeon, Minho Shong, Johan Auwerx, Dongryeol Ryu, Hyon‐Seung Yi
      Aging Cell.2020;[Epub]     CrossRef
    • miR-29a Modulates GSK3β/SIRT1-Linked Mitochondrial Proteostatic Stress to Ameliorate Mouse Non-Alcoholic Steatohepatitis
      Ya-Ling Yang, Pei-Wen Wang, Feng-Sheng Wang, Hung-Yu Lin, Ying-Hsien Huang
      International Journal of Molecular Sciences.2020; 21(18): 6884.     CrossRef
    • Impact of Mitophagy and Mitochondrial Unfolded Protein Response as New Adaptive Mechanisms Underlying Old Pathologies: Sarcopenia and Non-Alcoholic Fatty Liver Disease
      Rodrigo Urbina-Varela, Nataly Castillo, Luis A. Videla, Andrea del Campo
      International Journal of Molecular Sciences.2020; 21(20): 7704.     CrossRef
    • Molecular insights into the role of mitochondria in non-alcoholic fatty liver disease
      Jin Lee, Jeong-Su Park, Yoon Seok Roh
      Archives of Pharmacal Research.2019; 42(11): 935.     CrossRef
    • A siRNA mediated hepatic dpp4 knockdown affects lipid, but not glucose metabolism in diabetic mice
      Sven Wolfgang Görgens, Kerstin Jahn-Hofmann, Dinesh Bangari, Sheila Cummings, Christiane Metz-Weidmann, Uwe Schwahn, Paulus Wohlfart, Matthias Schäfer, Maximilian Bielohuby, Marcia B. Aguila
      PLOS ONE.2019; 14(12): e0225835.     CrossRef

    Figure

    Endocrinol Metab : Endocrinology and Metabolism