Fig. 1Effect of propylthiouracil (PTU) on high concentrations of iodide induced relative viability, lactate dehydrogenase (LDH) release and mitochondrial superoxide production in metallothionein I/II knockout (MT-I/II KO) and wild type (WT) mice. (A, B) Effects of PTU on high concentrations of iodide induced mitochondrial superoxide production. Histogram analysis was performed on the mean fluorescence intensity of MitoSOX Red as measured by flow cytometry. Experiments were repeated 3 times with similar results. (C) Effects of PTU on high concentrations of iodide induced LDH release. (D) Effects of PTU on high concentrations of iodide induced relative viability. One-way analysis of variance with a least significant difference test was used. aP<0.05 compared with the control group of WT or MT-I/II KO mice respectively; bP<0.05 compared with the potassium iodide (KI) group of WT or MT-I/II KO mice respectively; cP<0.05, WT mice compared with the MT-I/II KO mice under the same treatment.
Fig. 2Effect of perchlorate (KClO4) on high concentrations of iodide induced relative viability, lactate dehydrogenase (LDH) release and mitochondrial superoxide production in metallothionein I/II knockout (MT-I/II KO) and wild type (WT) mice. (A, B) Effects of KClO4 on high concentrations of iodide induced mitochondrial superoxide production. Histogram analysis was performed on the mean fluorescence intensity of MitoSOX Red as measured by flow cytometry. Experiments were repeated 3 times with similar results. (C) Effects of KClO4 on high concentrations of iodide induced LDH release. (D) Effects of KClO4 on high concentrations of iodide induced relative viability. One-way analysis of variance with a least significant difference test was used. aP<0.05 compared with the control group of WT or MT-I/II KO mice respectively; bP<0.05 compared with the potassium iodide (KI) group of WT or MT-I/II KO mice respectively; cP<0.05 WT mice compared with the MT-I/II KO mice under the same treatment.
Fig. 3Effect of thyroid stimulating hormone (TSH) on high concentrations of iodide induced relative viability, lactate dehydrogenase (LDH) release and mitochondrial superoxide production in metallothionein I/II knockout (MT-I/II KO) and wild type (WT) mice. (A, B) Effects of TSH on high concentrations of iodide induced mitochondrial superoxide production. Histogram analysis was performed on the mean fluorescence intensity of MitoSOX Red as measured by flow cytometry. Experiments were repeated 3 times with similar results. (C) Effects of TSH on high concentrations of iodide induced LDH release. (D) Effects of TSH on high concentrations of iodide induced relative viability. One-way analysis of variance with a least significant difference test was used. aP<0.05 compared with the control group of WT or MT-I/II KO mice respectively; bP<0.05 compared with the potassium iodide (KI) group of WT or MT-I/II KO mice respectively; cP<0.05 WT mice compared with the MT-I/II KO mice under the same treatment.