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Younghwa Kim  (Kim Y) 2 Articles
Polarized and Stage-Dependent Distribution of Immunoreactivity for Novel PDZ-Binding Protein Preso1 in Adult Neurogenic Regions
Eun Soo Lee, Woon Ryoung Kim, Younghwa Kim, Hyun Woo Lee, Hyun Kim, Woong Sun
Endocrinol Metab. 2014;29(3):349-355.   Published online September 25, 2014
DOI: https://doi.org/10.3803/EnM.2014.29.3.349
  • 3,529 View
  • 35 Download
  • 2 Web of Science
  • 1 Crossref
AbstractAbstract PDFPubReader   
Background

Adult neural stem cells have the potential for self-renewal and differentiation into multiple cell lineages via symmetric or asymmetric cell division. Preso1 is a recently identified protein involved in the formation of dendritic spines and the promotion of axonal growth in developing neurons. Preso1 can also bind to cell polarity proteins, suggesting a potential role for Preso1 in asymmetric cell division.

Methods

To investigate the distribution of Preso1, we performed immunohistochemistry with adult mouse brain slice. Also, polarized distribution of Preso1 was assessed by immunocytochemistry in cultured neural stem cells.

Results

Immunoreactivity for Preso1 (Preso1-IR) was strong in the rostral migratory stream and subventricular zone, where proliferating transit-amplifying cells and neuroblasts are prevalent. In cultured neural stem cells, Preso1-IR was unequally distributed in the cell cytosol. We also observed the distribution of Preso1 in the subgranular zone of the hippocampal dentate gyrus, another neurogenic region in the adult brain. Interestingly, Preso1-IR was transiently observed in the nuclei of doublecortin-expressing neuroblasts immediately after asymmetric cell division.

Conclusion

Our study demonstrated that Preso1 is asymmetrically distributed in the cytosol and nuclei of neural stem/progenitor cells in the adult brain, and may play a significant role in cell differentiation via association with cell polarity machinery.

Citations

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  • Articles in 'Endocrinology and Metabolism' in 2014
    Won-Young Lee
    Endocrinology and Metabolism.2015; 30(1): 47.     CrossRef
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Functional Role of Parkin against Oxidative Stress in Neural Cells
Minyoung Hwang, Ja-Myong Lee, Younghwa Kim, Dongho Geum
Endocrinol Metab. 2014;29(1):62-69.   Published online March 14, 2014
DOI: https://doi.org/10.3803/EnM.2014.29.1.62
  • 3,246 View
  • 28 Download
  • 5 Web of Science
  • 4 Crossref
AbstractAbstract PDFPubReader   
Background

Parkinson disease (PD) is caused by selective cell death of dopaminergic neurons in the substantia nigra. An early onset form of PD, autosomal recessive juvenile parkinsonism has been associated with a mutation in the parkin gene. The function of parkin is known to remove misfolding proteins and protect cell death. We aimed to investigate the role of parkin against oxidative stress in neuronal cells.

Methods

Parkin knockout embryonic stem cells (PKO ES cells) were differentiated into neurons by adherent monolayer culture method. Oxidative stress was induced by the treatment of 1-methyl-4-phenylpyridinium (MPP+) in neurons derived from wild type and PKO ES cells, and cell viability was examined by MTT assay. After exposure to MPP+, Tuj1-positive cell population was compared between PKO and wild type cells by fluorescence activated cell sorter (FACS) analysis. The activated caspase3 protein level was also measured by Western blot analysis, FACS and immunocytochemistry.

Results

There was no difference in the efficiency of neuronal differentiation between wild type and PKO ES cells. After exposure to MPP+, no significant differences were found in cell viability and Tuj1-positive cell population between the two groups determined by MTT assay and FACS analysis, respectively. The activated caspase3 protein levels examined by Western blot analysis, FACS and immunocytochemistry were not changed in PKO cells compared with those of wild type cells after MPP+ treatment.

Conclusion

These results suggest that PKO neuronal cells including dopaminergic neurons are not sensitive to caspase3-dependent cell death pathway during the response against MPP+-induced oxidative stress.

Citations

Citations to this article as recorded by  
  • miR-146b-5p promotes the neural conversion of pluripotent stem cells by targeting Smad4
    Nianping Zhang, Ying Lyu, Xuebing Pan, Liping Xu, Aiguo Xuan, Xiaosong He, Wandan Huang, Dahong Long
    International Journal of Molecular Medicine.2017; 40(3): 814.     CrossRef
  • Increased susceptibility to fundus camera-delivered light-induced retinal degeneration in mice deficient in oxidative stress response proteins
    Yi Ding, Bogale Aredo, Xin Zhong, Cynthia X. Zhao, Rafael L. Ufret-Vincenty
    Experimental Eye Research.2017; 159: 58.     CrossRef
  • Articles in 'Endocrinology and Metabolism' in 2014
    Won-Young Lee
    Endocrinology and Metabolism.2015; 30(1): 47.     CrossRef
  • Neural stem cells in Parkinson’s disease: a role for neurogenesis defects in onset and progression
    Jaclyn Nicole Le Grand, Laura Gonzalez-Cano, Maria Angeliki Pavlou, Jens C. Schwamborn
    Cellular and Molecular Life Sciences.2015; 72(4): 773.     CrossRef
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