- Diabetes
- In Vivo and In Vitro Quantification of Glucose Kinetics: From Bedside to Bench
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Il-Young Kim, Sanghee Park, Yeongmin Kim, Yewon Chang, Cheol Soo Choi, Sang-Hoon Suh, Robert R. Wolfe
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Endocrinol Metab. 2020;35(4):733-749. Published online December 23, 2020
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DOI: https://doi.org/10.3803/EnM.2020.406
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 Abstract
 PDF PubReader  ePub  Crossref - TDM
- Like other substrates, plasma glucose is in a dynamic state of constant turnover (i.e., rates of glucose appearance [Ra glucose] into and disappearance [Rd glucose] from the plasma) while staying within a narrow range of normal concentrations, a physiological priority. Persistent imbalance of glucose turnover leads to elevations (i.e., hyperglycemia, Ra>Rd) or falls (i.e., hypoglycemia, Ra<Rd) in the pool size, leading to clinical conditions such as diabetes. Endogenous Ra glucose is divided into hepatic glucose production via glycogenolysis and gluconeogenesis (GNG) and renal GNG. On the other hand, Rd glucose, the summed rate of glucose uptake by tissues/organs, involves various intracellular metabolic pathways including glycolysis, the tricarboxylic acid (TCA) cycle, and oxidation at varying rates depending on the metabolic status. Despite the dynamic nature of glucose metabolism, metabolic studies typically rely on measurements of static, snapshot information such as the abundance of mRNAs and proteins and (in)activation of implicated signaling networks without determining actual flux rates. In this review, we will discuss the importance of obtaining kinetic information, basic principles of stable isotope tracer methodology, calculations of in vivo glucose kinetics, and assessments of metabolic flux in experimental models in vivo and in vitro.
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Citations
Citations to this article as recorded by  - Essential Amino Acid-Enriched Diet Alleviates Dexamethasone-Induced Loss of Muscle Mass and Function through Stimulation of Myofibrillar Protein Synthesis and Improves Glucose Metabolism in Mice
Yeongmin Kim, Sanghee Park, Jinseok Lee, Jiwoong Jang, Jiyeon Jung, Jin-Ho Koh, Cheol Soo Choi, Robert R. Wolfe, Il-Young Kim
Metabolites.2022; 12(1): 84. CrossRef - Exploring Human Muscle Dynamics In Vivo Using Stable Isotope Tracers
Il-Young Kim, Sanghee Park, Jiwoong Jang, Yeongmin Kim, Hee-Joo Kim
Annals of Clinical Nutrition and Metabolism.2022; 14(2): 40. CrossRef
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