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Endocrinol Metab : Endocrinology and Metabolism


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Won Ho Kim  (Kim WH) 2 Articles
Endocrine Research
Diastolic Dysfunction Induced by a High-Fat Diet Is Associated with Mitochondrial Abnormality and Adenosine Triphosphate Levels in Rats
Ki-Woon Kang, Ok-Soon Kim, Jung Yeon Chin, Won Ho Kim, Sang Hyun Park, Yu Jeong Choi, Jong Ho Shin, Kyung Tae Jung, Do-Seon Lim, Seong-Kyu Lee
Endocrinol Metab. 2015;30(4):557-568.   Published online December 31, 2015
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  • 37 Download
  • 10 Citations
AbstractAbstract PDFPubReader   CrossRef-TDMCrossref - TDM

Obesity is well-known as a risk factor for heart failure, including diastolic dysfunction. However, this mechanism in high-fat diet (HFD)-induced obese rats remain controversial. The purpose of this study was to investigate whether cardiac dysfunction develops when rats are fed with a HFD for 10 weeks; additionally, we sought to investigate the association between mitochondrial abnormalities, adenosine triphosphate (ATP) levels and cardiac dysfunction.


We examined myocardia in Wistar rats after 10 weeks of HFD (45 kcal% fat, n=6) or standard diet (SD, n=6). Echocardiography, histomorphologic analysis, and electron microscopy were performed. The expression levels of mitochondrial oxidative phosphorylation (OXPHOS) subunit genes, peroxisome-proliferator-activated receptor γ co-activator-1α (PGC1α) and anti-oxidant enzymes were assessed. Markers of oxidative stress damage, mitochondrial DNA copy number and myocardial ATP level were also examined.


After 10 weeks, the body weight of the HFD group (349.6±22.7 g) was significantly higher than that of the SD group (286.8±14.9 g), and the perigonadal and epicardial fat weights of the HFD group were significantly higher than that of the SD group. Histomorphologic and electron microscopic images were similar between the two groups. However, in the myocardium of the HFD group, the expression levels of OXPHOS subunit NDUFB5 in complex I and PGC1α, and the mitochondrial DNA copy number were decreased and the oxidative stress damage marker 8-hydroxydeoxyguanosine was increased, accompanied by reduced ATP levels.


Diastolic dysfunction was accompanied by the mitochondrial abnormality and reduced ATP levels in the myocardium of 10 weeks-HFD-induced rats.


Citations to this article as recorded by  
  • Surgically Metabolic Resection of Pericardial Fat to Ameliorate Myocardial Mitochondrial Dysfunction in Acute Myocardial Infarction Obese Rats
    Ki-Woon Kang, Ju-Young Ko, Hyunghee Lee, Seung Yong Shin, Wang Soo Lee, Joonhwa Hong, Sang-Wook Kim, Seong-Kyu Lee, Min-Ho Oak
    Journal of Korean Medical Science.2022;[Epub]     CrossRef
  • Cilostazol attenuates cardiac oxidative stress and inflammation in hypercholesterolemic rats
    Rosane de Oliveira Lopes, Gabriel Ferreira Lima, Ana Beatriz Araújo Mendes, Lis Jappour Autran, Nikolas Cunha de Assis Pereira, Stephani Correia Brazão, Beatriz Alexandre-Santos, Eliete Dalla Corte Frantz, Christianne Brêtas Vieira Scaramello, Fernanda Ca
    Naunyn-Schmiedeberg's Archives of Pharmacology.2022; 395(7): 789.     CrossRef
  • The impact of diet upon mitochondrial physiology (Review)
    Ioannis Kyriazis, Eleni Vassi, Maria Alvanou, Christos Angelakis, Zoi Skaperda, Fotios Tekos, Venkata Garikipati, Demetrios Spandidos, Demetrios Kouretas
    International Journal of Molecular Medicine.2022;[Epub]     CrossRef
  • SIRT1 promotes lipid metabolism and mitochondrial biogenesis in adipocytes and coordinates adipogenesis by targeting key enzymatic pathways
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    Thembeka A. Nyawo, Carmen Pheiffer, Sithandiwe E. Mazibuko-Mbeje, Sinenhlanhla X. H. Mthembu, Tawanda M. Nyambuya, Bongani B. Nkambule, Hanél Sadie-Van Gijsen, Hans Strijdom, Luca Tiano, Phiwayinkosi V. Dludla
    Antioxidants.2021; 10(11): 1758.     CrossRef
  • Potentially Critical Roles ofNDUFB5,TIMMDC1,and VDAC3in the Progression of Septic Cardiomyopathy Through Integrated Bioinformatics Analysis
    Kai Kang, Jingtian Li, Ruidong Li, Xiufeng Xu, Jianli Liu, Limin Qin, Tao Huang, Jinhua Wu, Min Jiao, Miaomiao Wei, Hongjie Wang, Tao Wang, Quan Zhang
    DNA and Cell Biology.2020; 39(1): 105.     CrossRef
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    François Briand, Emmanuel Brousseau, Julie Maupoint, Caroline Dubroca, Clément Costard, Natalia Breyner, Rémy Burcelin, Thierry Sulpice
    European Journal of Pharmacology.2020; 882: 173316.     CrossRef
  • Mitochondrial impairment following neonatal overfeeding: A comparison between normal and ischemic‐reperfused hearts
    Cristiane de Moura Freitas, Luciana Caroline Paulino do Nascimento, Glauber Rudá Feitoza Braz, Severina Cassia Andrade‐Silva, Nelson C. Lima‐Junior, Tercya de Araujo Silva, Mariana Pinheiro Fernandes, Diorginis José Soares Ferreira, Claudia Jacques Lagran
    Journal of Cellular Biochemistry.2019; 120(5): 7341.     CrossRef
  • Exercise training reverses age-induced diastolic dysfunction and restores coronary microvascular function
    Kazuki Hotta, Bei Chen, Bradley J. Behnke, Payal Ghosh, John N. Stabley, Jeremy A. Bramy, Jaime L. Sepulveda, Michael D. Delp, Judy M. Muller-Delp
    The Journal of Physiology.2017; 595(12): 3703.     CrossRef
  • Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats
    Jingfeng Wang, Zhiming Li, Yanyan Wang, Jingjing Zhang, Weipeng Zhao, Mingqiang Fu, Xueting Han, Jingmin Zhou, Junbo Ge
    Evidence-Based Complementary and Alternative Medicine.2017; 2017: 1.     CrossRef
Association of Polymorphism in beta3-Adrenergic Receptor Gene with Fat Distribution.
Tae Sung Yun, Yong Deuk Kim, Hye Soon Kim, Mi Jung Kim, Young Sung Suh, Jung Hyeok Kwon, Jin Soo Choi, Jung Guk Kim, Sung Woo Ha, Bo Wan Kim, Kyu Chang Won, Hyong Woo Lee, Ho Sang Shon, Ji Hyun Lee, Hyun Dae Yoon, Won Ho Kim, Young Gil Yun, In Kyu Lee
J Korean Endocr Soc. 2003;18(2):184-192.   Published online April 1, 2003
  • 1,078 View
  • 16 Download
AbstractAbstract PDF
Reasons for obesity include environmental factors and, more largely so, genetic factors. There have been many studies on these genetic factors. So far, genes related to obesity such as Leptin, Uncoupling Protein(UCP), Peroxisome proliferator activated receptor-gamma(PPAR-gamma), and Beta3-adrener-gic receptor(beta3-AR) gene have been discovered. Among these, beta3-AR is expressed in visceral adipose tissue and is thought to contribute to the regulation of resting metabolic rate and lipolysis. The missense mutation of beta3-AR gene, resulting in replacement of tryptophan by arginine at position 64(Trp64Arg), is associated with decreased resting metabolic rate and weightgain. We performed this study to determine if Trp64Arg polymorphism of beta3-AR gene is associatedwith obesity in Koreans. METHOD: We investigated the relationship between the beta3-AR gene mutation and body mass index (BMI), waist circumference, hip circumference, waist to hip ratio(WHR), area of subcutaneous fat, area of visceral fat, visceral to subcutaneous fat ratio(VSR), and lipid profile. 198 subjects were included in this study of which 97 were of normal weight and 101 were obese. Anthropometric data was obtained from physical examination and medical records. RESULT: In the cases of beta3-AR gene mutation of the obese group, the ratio of Trp/Arg and Arg/Arg are 43% and 5%, respectively, which were higher than the normal group(36%, 1%), although a statistical significant was not found. There was significant difference in the are of subcutaneous fat. Normal group(Trp/Trp) measured at 213.9+/-109.6cm2 versus 244.0+/-127.7cm2 (Trp/Arg) and 323.9+/-189.9cm2(Arg/Arg) for the mutation groups. Circumference of waist, circumference of hip, WHR, area of visceral fat, and VSR were higher in the mutation groups than in normal subject, but not significantly different. CONCLUSION: These results suggest that a genetic mutation in the beta3-AR gene can affect body fat composition, and is associated with obesity in Korean adults.

Endocrinol Metab : Endocrinology and Metabolism