Endocrinology and Metabolism

Kunsoo Rhee (Rhee K)

1 Article

Determination of Mother Centriole Maturation in CPAP-Depleted Cells Using the Ninein Antibody: Miseon Lee, Kunsoo Rhee; Endocrinol Metab. 2015;30(1):53-57. Published online March 27, 2015; DOI: https://doi.org/10.3803/EnM.2015.30.1.53

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Background

Mutations in centrosomal protein genes have been identified in a number of genetic diseases in brain development, including microcephaly. Centrosomal P4.1-associated protein (CPAP) is one of the causal genes implicated in primary microcephaly. We previously proposed that CPAP is essential for mother centriole maturation during mitosis.

Methods

We immunostained CPAP-depleted cells using the ninein antibody, which selectively detects subdistal appendages in mature mother centrioles.

Results

Ninein signals were significantly impaired in CPAP-depleted cells.

Conclusion

The results suggest that CPAP is required for mother centriole maturation in mammalian cells. The selective absence of centriolar appendages in young mother centrioles may be responsible for asymmetric spindle pole formation in CPAP-depleted cells.

Citations

Citations to this article as recorded by

The intercentriolar fibers function as docking sites of centriolar satellites for cilia assembly
Sungjin Ryu, Donghee Ko, Byungho Shin, Kunsoo Rhee
Journal of Cell Biology.2024;[Epub] CrossRef
CPAP insufficiency leads to incomplete centrioles that duplicate but fragment
Alejandra Vásquez-Limeta, Kimberly Lukasik, Dong Kong, Catherine Sullenberger, Delgermaa Luvsanjav, Natalie Sahabandu, Raj Chari, Jadranka Loncarek
Journal of Cell Biology.2022;[Epub] CrossRef
Loss of CPAP causes sustained EGFR signaling and epithelial-mesenchymal transition in oral cancer
Radhika R. Gudi, Harinarayanan Janakiraman, Philip H. Howe, Viswanathan Palanisamy, Chenthamarakshan Vasu
Oncotarget.2021; 12(8): 807. CrossRef
NANOG/NANOGP8 Localizes at the Centrosome and is Spatiotemporally Associated with Centriole Maturation
Erika Mikulenkova, Jakub Neradil, Ondrej Vymazal, Jan Skoda, Renata Veselska
Cells.2020; 9(3): 692. CrossRef
ODF2 maintains centrosome cohesion by restricting β-catenin accumulation
Kefei Yang, Marco Andreas Tylkowski, Daniela Hüber, Constanza Tapia Contreras, Sigrid Hoyer-Fender
Journal of Cell Science.2018;[Epub] CrossRef

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