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Jung Soo Kim  (Kim JS) 2 Articles
Characteristics of Serum Insulin-like Growth Factor ( IGF ) and IGF-Bindign Protein-3 during Pregnancy.
Dae Yeol Lee, Jung Soo Kim, Hong Ro Lee, Cheol Hee Rhee, Soo Chul Cho
J Korean Endocr Soc. 1997;12(3):376-385.   Published online January 1, 2001
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  • 18 Download
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BACKGROUND
Pregnancy in human and rodents is associated with dramatic matemal metabolic changes. Insulin-like growth factors (IGFs) are mitogenic peptides that are essential for fetal and maternal tissue growth during pregnancy. They circulate complexed primarily with a serum IGF-binding protein (IGFBP-3) which regulates the availability of the IGFs to their specific target tissues. METHODS: To examine the changes of IGFs and IGFB-3 during pregnancy, we measured serum total IGF-I, free IGF-I, IGF-II and IGFBP-3 by using specific radioimmunoassay, immunoradio-metric assay, western ligand blot and western immunoblot. Blood samples were obtained from 88 pregnant women between 6-40 weeks gestation. RESULTS: While serum IGF-I levels increased up to 50% in late pregnancy, serum IGF-II levels remained unchanged. However, serum free IGF-I levels were significantly higher during pregnancy than in nonpregnancy. Western ligand blot analysis revealed that IGFBP-3 in pregnancy serum was significantly decreased at 6 weeks of gestation, continued decreased level until term, and returned to a nonpregnant level by postpartum 10 day. Serum IGFBP-3 profiles in Western immunoblot analysis revealed that 30 kDa fragments of IGFBP-3 were detectable in pregnancy serum but not in nonpregnancy serum. In contrast, serum IGFBP-3 levels using radioimmunoassay was significantly increased in late pregnancy. CONCLUSIONS: 1) serum IGF-I was significantly elevated in late pregnancy 2) serum IGF-II was not significantly changed 3) free IGF-I significantly elevated throughout gestation 4) intact IGFBP-3 was markedly reduced after 6 weeks of gestation.
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Ca Effects on Synthesis and Secretion of Insulin-like Growth Factor(IGF-I) and IGF-Binding Proteins by the Perfased Rat Liver.
Dae Yeol Lee, Chang Won Kang, Jung Soo Kim
J Korean Endocr Soc. 1996;11(2):189-198.   Published online November 7, 2019
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  • 27 Download
AbstractAbstract PDF
Background
The insulin-like growth factors, IGF-I and -II, are important metabolic factors involved in cell growth and metabolism. The IGFs are produced in most organs but the liver is believed to be the principal source of circulating IGF-I. It has been demonstrated that calcium in the extracellular fluid has effects on the secretion of various hormones such as parathyroid hormone, insulin and atrial natriuretic peptide in variety of tissues. Methods: In arder to investigate that liver produce IGF-1 and IGFBP-3 and the role of calcium in the regulation of IGF-I and IGFBP-3 secretion and synthesis, the rat liver perfusian model was employed. The liver was perfused with Krebs-Henseleit bicarbonate(KHB) buffer containing 0 or 2.5 mM CaC12 for 2 hours, and 4-ml fractions of perfusates were collected and determined the concentration of IGF-I and IGFBP-3 by using RIA after Sep-pak extraction and Western ligand blot. respectively. To increase or decrease the concentration of intracellular calcium, we also used EGTA, calciurn ionophore A23187 increased IGF-I secretion and synthesis in liver(18.13+0.97 vs 15.78+1.01, p<0.01). However, concentration of glucose was not significantly affected by both calcium(2.07+1.44 vs 2.24+1.74) and BGTA(2.01+1A7 vs 3.11+1.01). Conclusion: Our results demonstrate that liver is a major place far IGF-I and IOFBP-3 production and calcium specifically affects the secretion of IGF-I in the liver perfusion, suggesting that the calcium environment of hepatic cells may influence the secretion of the hepatic IGF-I.
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