Skip Navigation
Skip to contents

Endocrinol Metab : Endocrinology and Metabolism


Author index

Page Path
Ji-Won Kim 1 Article
Endocrine Research
Suppression of Fibrotic Reactions of Chitosan-Alginate Microcapsules Containing Porcine Islets by Dexamethasone Surface Coating
Min Jung Kim, Heon-Seok Park, Ji-Won Kim, Eun-Young Lee, Marie Rhee, Young-Hye You, Gilson Khang, Chung-Gyu Park, Kun-Ho Yoon
Endocrinol Metab. 2021;36(1):146-156.   Published online February 24, 2021
  • 5,260 View
  • 146 Download
  • 9 Citations
AbstractAbstract PDFPubReader   ePub   
The microencapsulation is an ideal solution to overcome immune rejection without immunosuppressive treatment. Poor biocompatibility and small molecular antigens secreted from encapsulated islets induce fibrosis infiltration. Therefore, the aims of this study were to improve the biocompatibility of microcapsules by dexamethasone coating and to verify its effect after xenogeneic transplantation in a streptozotocin-induced diabetes mice.
Dexamethasone 21-phosphate (Dexa) was dissolved in 1% chitosan and was cross-linked with the alginate microcapsule surface. Insulin secretion and viability assays were performed 14 days after microencapsulation. Dexa-containing chitosan-coated alginate (Dexa-chitosan) or alginate microencapsulated porcine islets were transplanted into diabetic mice. The fibrosis infiltration score was calculated from the harvested microcapsules. The harvested microcapsules were stained with trichrome and for insulin and macrophages.
No significant differences in glucose-stimulated insulin secretion and islet viability were noted among naked, alginate, and Dexa-chitosan microencapsulated islets. After transplantation of microencapsulated porcine islets, nonfasting blood glucose were normalized in both the Dexa-chitosan and alginate groups until 231 days. The average glucose after transplantation were lower in the Dexa-chitosan group than the alginate group. Pericapsular fibrosis and inflammatory cell infiltration of microcapsules were significantly reduced in Dexa-chitosan compared with alginate microcapsules. Dithizone and insulin were positive in Dexa-chitosan capsules. Although fibrosis and macrophage infiltration was noted on the surface, some alginate microcapsules were stained with insulin.
Dexa coating on microcapsules significantly suppressed the fibrotic reaction on the capsule surface after transplantation of xenogenic islets containing microcapsules without any harmful effects on the function and survival of the islets.


Citations to this article as recorded by  
  • Engineering superstable islets-laden chitosan microgels with carboxymethyl cellulose coating for long-term blood glucose regulation in vivo
    Haofei Li, Weijun He, Qi Feng, Junlin Chen, Xinbin Xu, Chuhan Lv, Changchun Zhu, Hua Dong
    Carbohydrate Polymers.2024; 323: 121425.     CrossRef
  • A Case for Material Stiffness as a Design Parameter in Encapsulated Islet Transplantation
    Courtney D. Johnson, Helim Aranda-Espinoza, John P. Fisher
    Tissue Engineering Part B: Reviews.2023; 29(4): 334.     CrossRef
  • Improved membrane stability of alginate-chitosan microcapsules by crosslinking with tannic acid
    Li Chen, Fang Jiang, Haidan Xu, Yaoyao Fan, Cunbin Du
    Biotechnology Letters.2023; 45(8): 1039.     CrossRef
  • Advances in alginate encapsulation of pancreatic islets for immunoprotection in type 1 diabetes
    Dinesh Chaudhary, Tiep Tien Nguyen, Simmyung Yook, Jee-Heon Jeong
    Journal of Pharmaceutical Investigation.2023; 53(5): 601.     CrossRef
  • Investigation of encapsulation of pancreatic beta cells and curcumin within alginate microcapsules
    Zahra Hosseinzadeh, Iran Alemzadeh, Manouchehr Vossoughi
    The Canadian Journal of Chemical Engineering.2023;[Epub]     CrossRef
  • Emerging strategies for beta cell transplantation to treat diabetes
    Jesus Paez-Mayorga, Izeia Lukin, Dwaine Emerich, Paul de Vos, Gorka Orive, Alessandro Grattoni
    Trends in Pharmacological Sciences.2022; 43(3): 221.     CrossRef
  • Layer-by-Layer Cell Encapsulation for Drug Delivery: The History, Technique Basis, and Applications
    Wenyan Li, Xuejiao Lei, Hua Feng, Bingyun Li, Jiming Kong, Malcolm Xing
    Pharmaceutics.2022; 14(2): 297.     CrossRef
  • β cell replacement therapy for the cure of diabetes
    Joonyub Lee, Kun‐Ho Yoon
    Journal of Diabetes Investigation.2022; 13(11): 1798.     CrossRef
  • Modern pancreatic islet encapsulation technologies for the treatment of type 1 diabetes
    P. S. Ermakova, E. I. Cherkasova, N. A. Lenshina, A. N. Konev, M. A. Batenkin, S. A. Chesnokov, D. M. Kuchin, E. V. Zagainova, V. E. Zagainov, A. V. Kashina
    Russian Journal of Transplantology and Artificial Organs.2021; 23(4): 95.     CrossRef
Close layer

Endocrinol Metab : Endocrinology and Metabolism