- Gene Expression Regulation by Agonist-Independent Constitutive Signaling of Melanocortin-1 Receptor
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Ikjoo Seong, Jaesang Kim
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Endocrinol Metab. 2014;29(2):179-184. Published online June 26, 2014
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DOI: https://doi.org/10.3803/EnM.2014.29.2.179
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Abstract
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- Background
Melanocortin-1 receptor (Mc1r), a key signaling receptor for melanogenesis, has been reported to mediate migration of B16F10 melanoma cells. Interestingly, this activity appears to be a part of the constitutive signaling of Mc1r. MethodsWe carried out small interfering RNA-mediated knock-down of Mc1r on murine melanoma B16F10 cells and performed microarray analysis to characterize changes in the gene expression profile. ResultsWe isolated 22 and four genes whose expression decreased and increased, respectively, by 2.5-fold or higher as the result of Mc1r knock-down. Several down-regulated genes have been proposed to be involved in cell migration. Among these genes are several members of the chemokine gene family. ConclusionWe provide a gene set for further functional analyses of Mc1r. The Mc1r target genes we present may be particularly relevant for understanding the ligand-independent activity of Mc1r. Further examination of the mode of action may lead to novel strategies in regulating the migration and metastasis of melanoma cells.
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Citations
Citations to this article as recorded by 
- NRF2 and Key Transcriptional Targets in Melanoma Redox Manipulation
Evan L. Carpenter, Alyssa L. Becker, Arup K. Indra Cancers.2022; 14(6): 1531. CrossRef - Hormonal Regulation of the Repair of UV Photoproducts in Melanocytes by the Melanocortin Signaling Axis
Stuart G. Jarrett, John A. D'Orazio Photochemistry and Photobiology.2017; 93(1): 245. CrossRef - Articles in 'Endocrinology and Metabolism' in 2014
Won-Young Lee Endocrinology and Metabolism.2015; 30(1): 47. CrossRef
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