Skip Navigation
Skip to contents

Endocrinol Metab : Endocrinology and Metabolism


Author index

Page Path
Iruni Roshanie Abeysekera  (Abeysekera IR) 1 Article
Endocrine Research
Propylthiouracil, Perchlorate, and Thyroid-Stimulating Hormone Modulate High Concentrations of Iodide Instigated Mitochondrial Superoxide Production in the Thyroids of Metallothionein I/II Knockout Mice
Qi Duan, Tingting Wang, Na Zhang, Vern Perera, Xue Liang, Iruni Roshanie Abeysekera, Xiaomei Yao
Endocrinol Metab. 2016;31(1):174-184.   Published online March 16, 2016
  • 4,456 View
  • 37 Download
  • 6 Citations
AbstractAbstract PDFPubReader   

Increased oxidative stress has been suggested as one of the underlying mechanisms in iodide excess-induced thyroid disease. Metallothioneins (MTs) are regarded as scavengers of reactive oxygen species (ROS) in oxidative stress. Our aim is to investigate the effects of propylthiouracil (PTU), a thyroid peroxidase inhibitor, perchlorate (KClO4), a competitive inhibitor of iodide transport, and thyroid stimulating hormone (TSH) on mitochondrial superoxide production instigated by high concentrations of iodide in the thyroids of MT-I/II knockout (MT-I/II KO) mice.


Eight-week-old 129S7/SvEvBrd-Mt1tm1Bri Mt2tm1Bri/J (MT-I/II KO) mice and background-matched wild type (WT) mice were used.


By using a mitochondrial superoxide indicator (MitoSOX Red), lactate dehydrogenase (LDH) release, and methyl thiazolyl tetrazolium (MTT) assay, we demonstrated that the decreased relative viability and increased LDH release and mitochondrial superoxide production induced by potassium iodide (100 µM) can be relieved by 300 µM PTU, 30 µM KClO4, or 10 U/L TSH in the thyroid cell suspensions of both MT-I/II KO and WT mice (P<0.05). Compared to the WT mice, a significant decrease in the relative viability along with a significant increase in LDH release and mitochondrial superoxide production were detected in MT-I/II KO mice(P<0.05).


We concluded that PTU, KClO4, or TSH relieved the mitochondrial oxidative stress induced by high concentrations of iodide in the thyroids of both MT-I/II KO and WT mice. MT-I/II showed antioxidant effects against high concentrations of iodide-induced mitochondrial superoxide production in the thyroid.


Citations to this article as recorded by  
  • The reversal of drug resistance by two‐dimensional titanium carbide Ti2C (2D Ti2C) in non‐small‐cell lung cancer via the depletion of intracellular antioxidant reserves
    Yue Zhu, Baiyan Sui, Xin Liu, Jiao Sun
    Thoracic Cancer.2021; 12(24): 3340.     CrossRef
  • Thyroid function alterations attributed to high iodide supplementation in maternal rats and their offspring
    Xue Liang, Yanni Feng, Laixiang Lin, Iruni Roshanie Abeysekera, Umar Iqbal, Tingting Wang, Ying Wang, Xiaomei Yao
    Journal of Trace Elements in Medicine and Biology.2018; 47: 89.     CrossRef
  • Ecotoxicological assessment of perchlorate using in vitro and in vivo assays
    Rosa Acevedo-Barrios, Consuelo Sabater-Marco, Jesus Olivero-Verbel
    Environmental Science and Pollution Research.2018; 25(14): 13697.     CrossRef
  • Isolated domains of recombinant human apo-metallothionein 1A are folded at neutral pH: a denaturant and heat-induced unfolding study using ESI-MS
    Gordon W. Irvine, Natalie Korkola, Martin J. Stillman
    Bioscience Reports.2018;[Epub]     CrossRef
  • Reappraisal of metallothionein: Clinical implications for patients with diabetes mellitus
    Yongsoo Park, Jian Zhang, Lu Cai
    Journal of Diabetes.2018; 10(3): 213.     CrossRef
  • Mammalian Metallothionein-2A and Oxidative Stress
    Xue-Bin Ling, Hong-Wei Wei, Jun Wang, Yue-Qiong Kong, Yu-You Wu, Jun-Li Guo, Tian-Fa Li, Ji-Ke Li
    International Journal of Molecular Sciences.2016; 17(9): 1483.     CrossRef
Close layer

Endocrinol Metab : Endocrinology and Metabolism