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Dongho Geum  (Geum D) 1 Article
Functional Role of Parkin against Oxidative Stress in Neural Cells
Minyoung Hwang, Ja-Myong Lee, Younghwa Kim, Dongho Geum
Endocrinol Metab. 2014;29(1):62-69.   Published online March 14, 2014
DOI: https://doi.org/10.3803/EnM.2014.29.1.62
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  • 6 Web of Science
  • 5 Crossref
AbstractAbstract PDFPubReader   
Background

Parkinson disease (PD) is caused by selective cell death of dopaminergic neurons in the substantia nigra. An early onset form of PD, autosomal recessive juvenile parkinsonism has been associated with a mutation in the parkin gene. The function of parkin is known to remove misfolding proteins and protect cell death. We aimed to investigate the role of parkin against oxidative stress in neuronal cells.

Methods

Parkin knockout embryonic stem cells (PKO ES cells) were differentiated into neurons by adherent monolayer culture method. Oxidative stress was induced by the treatment of 1-methyl-4-phenylpyridinium (MPP+) in neurons derived from wild type and PKO ES cells, and cell viability was examined by MTT assay. After exposure to MPP+, Tuj1-positive cell population was compared between PKO and wild type cells by fluorescence activated cell sorter (FACS) analysis. The activated caspase3 protein level was also measured by Western blot analysis, FACS and immunocytochemistry.

Results

There was no difference in the efficiency of neuronal differentiation between wild type and PKO ES cells. After exposure to MPP+, no significant differences were found in cell viability and Tuj1-positive cell population between the two groups determined by MTT assay and FACS analysis, respectively. The activated caspase3 protein levels examined by Western blot analysis, FACS and immunocytochemistry were not changed in PKO cells compared with those of wild type cells after MPP+ treatment.

Conclusion

These results suggest that PKO neuronal cells including dopaminergic neurons are not sensitive to caspase3-dependent cell death pathway during the response against MPP+-induced oxidative stress.

Citations

Citations to this article as recorded by  
  • A Modified Differentiation Protocol In Vitro to Generate Dopaminergic Neurons from Pluripotent Stem Cells
    Nianping Zhang, Xudong Zhang, Zhaoli Yan, Ronghui Li, Song Xue, Dahong Long
    Journal of Biomaterials and Tissue Engineering.2023; 13(10): 1017.     CrossRef
  • miR-146b-5p promotes the neural conversion of pluripotent stem cells by targeting Smad4
    Nianping Zhang, Ying Lyu, Xuebing Pan, Liping Xu, Aiguo Xuan, Xiaosong He, Wandan Huang, Dahong Long
    International Journal of Molecular Medicine.2017; 40(3): 814.     CrossRef
  • Increased susceptibility to fundus camera-delivered light-induced retinal degeneration in mice deficient in oxidative stress response proteins
    Yi Ding, Bogale Aredo, Xin Zhong, Cynthia X. Zhao, Rafael L. Ufret-Vincenty
    Experimental Eye Research.2017; 159: 58.     CrossRef
  • Articles in 'Endocrinology and Metabolism' in 2014
    Won-Young Lee
    Endocrinology and Metabolism.2015; 30(1): 47.     CrossRef
  • Neural stem cells in Parkinson’s disease: a role for neurogenesis defects in onset and progression
    Jaclyn Nicole Le Grand, Laura Gonzalez-Cano, Maria Angeliki Pavlou, Jens C. Schwamborn
    Cellular and Molecular Life Sciences.2015; 72(4): 773.     CrossRef
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