Skip Navigation
Skip to contents

Endocrinol Metab : Endocrinology and Metabolism

clarivate
OPEN ACCESS
SEARCH
Search

Author index

Page Path
HOME > BROWSE ARTICLES > Author index
Search
Dong-Gyu Kim  (Kim DG) 1 Article
A Novel Cytosolic Isoform of Mitochondrial Trans-2-Enoyl-CoA Reductase Enhances Peroxisome Proliferator-Activated Receptor α Activity
Dong-Gyu Kim, Jae Cheal Yoo, Eunju Kim, Young-Sun Lee, Oleg V. Yarishkin, Da Yong Lee, Kun Ho Lee, Seong-Geun Hong, Eun Mi Hwang, Jae-Yong Park
Endocrinol Metab. 2014;29(2):185-194.   Published online June 26, 2014
DOI: https://doi.org/10.3803/EnM.2014.29.2.185
  • 5,012 View
  • 47 Download
  • 20 Web of Science
  • 21 Crossref
AbstractAbstract PDFPubReader   
Background

Mitochondrial trans-2-enoyl-CoA reductase (MECR) is involved in mitochondrial synthesis of fatty acids and is highly expressed in mitochondria. MECR is also known as nuclear receptor binding factor-1, which was originally reported with yeast two-hybrid screening as a binding protein of the nuclear hormone receptor peroxisome proliferator-activated receptor α (PPARα). However, MECR and PPARα are localized at different compartment, mitochondria, and the nucleus, respectively. Therefore, the presence of a cytosolic or nuclear isoform of MECR is necessary for functional interaction between MECR and PPARα.

Methods

To identify the expression pattern of MECR and the cytosolic form of MECR (cMECR), we performed reverse transcription polymerase chain reaction (RT-PCR) with various tissue samples from Sprague-Dawley rats. To confirm the interaction between cMECR and PPARα, we performed several binding assays such as yeast two-hybrid, coimmunoprecipitation, and bimolecular fluorescence complementation. To observe subcellular localization of these proteins, immunocytochemistry was performed. A luciferase assay was used to measure PPARα activity.

Results

We provide evidence of an alternatively spliced variant of the rat MECR gene that yields cMECR. The cMECR lacks the N-terminal 76 amino acids of MECR and shows uniform distribution in the cytoplasm and nucleus of HeLa cells. cMECR directly bound PPARα in the nucleus and increased PPARα-dependent luciferase activity in HeLa cells.

Conclusion

We found the cytosolic form of MECR (cMECR) was expressed in the cytosolic and/or nuclear region, directly binds with PPARα, and enhances PPARα activity.

Citations

Citations to this article as recorded by  
  • Metabolism of phenolics in coffee and plant-based foods by canonical pathways: an assessment of the role of fatty acid β-oxidation to generate biologically-active and -inactive intermediates
    Michael N. Clifford, Laurence J. King, Asimina Kerimi, Maria Gema Pereira-Caro, Gary Williamson
    Critical Reviews in Food Science and Nutrition.2024; 64(11): 3326.     CrossRef
  • Comparison of muscle nutritional composition, texture quality, carotenoid metabolites and transcriptome to underling muscle quality difference between wild-caught and pond-cultured Yellow River carp (Cyprinus carpio haematopterus)
    Luming Wang, Jinrui Xiong, Chunchu Xu, Chaobin Qin, Yuru Zhang, Liping Yang, Shaoyang Zhi, Jianxin Feng, Guoxing Nie
    Aquaculture.2024; 581: 740392.     CrossRef
  • Effects of microcystin-LR on immune function, lipid metabolism and intestinal microbial structure in Eriocheir sinensis
    Jinliang Du, Liping Cao, Jiancao Gao, Zhijuan Nie, Quanjie Li, Yi Sun, Nailin Shao, Jiawen Hu, Lin Zhou, Guojun Yin, Gangchun Xu
    Aquaculture Reports.2024; 35: 101994.     CrossRef
  • A defect in mitochondrial fatty acid synthesis impairs iron metabolism and causes elevated ceramide levels
    Debdeep Dutta, Oguz Kanca, Seul Kee Byeon, Paul C. Marcogliese, Zhongyuan Zuo, Rishi V. Shridharan, Jun Hyoung Park, Guang Lin, Ming Ge, Gali Heimer, Jennefer N. Kohler, Matthew T. Wheeler, Benny A. Kaipparettu, Akhilesh Pandey, Hugo J. Bellen
    Nature Metabolism.2023; 5(9): 1595.     CrossRef
  • Alternative splicing liberates a cryptic cytoplasmic isoform of mitochondrial MECR that antagonizes influenza virus
    Steven F. Baker, Helene Meistermann, Manuel Tzouros, Aaron Baker, Sabrina Golling, Juliane Siebourg Polster, Mitchell P. Ledwith, Anthony Gitter, Angelique Augustin, Hassan Javanbakht, Andrew Mehle, Frank Kirchhoff
    PLOS Biology.2022; 20(12): e3001934.     CrossRef
  • Genetic variants in ALDH1L1 and GLDC influence the serine-to-glycine ratio in Hispanic children
    Sergey A Krupenko, Shelley A Cole, Ruixue Hou, Karin Haack, Sandra Laston, Nitesh R Mehta, Anthony G Comuzzie, Nancy F Butte, V Saroja Voruganti
    The American Journal of Clinical Nutrition.2022; 116(2): 500.     CrossRef
  • Simultaneous Presentation of Multiple Myeloma and Lung Cancer: Case Report and Gene Bioinformatics Analysis
    Ping-Ping Xiao, Bing-Qing Luo, Wei Fan, Xu-Yan Chen, Zhi-Gao Dong, Jin-Mei Huang, Yi Zhang, Yong-Quan Chen
    Frontiers in Oncology.2022;[Epub]     CrossRef
  • Fatty acid metabolism-related genes are associated with flavor-presenting aldehydes in Chinese local chicken
    Xiaoya Yuan, Huanxian Cui, Yuxi Jin, Wenjuan Zhao, Xiaojing Liu, Yongli Wang, Jiqiang Ding, Li Liu, Jie Wen, Guiping Zhao
    Frontiers in Genetics.2022;[Epub]     CrossRef
  • NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma
    Jeongha Kim, Hyunkoo Kang, Beomseok Son, Min-Jung Kim, JiHoon Kang, Kang Hyun Park, Jaewan Jeon, Sunmi Jo, Hae Yu Kim, HyeSook Youn, BuHyun Youn
    Experimental & Molecular Medicine.2022; 54(11): 1872.     CrossRef
  • Mitochondrial Fatty Acids and Neurodegenerative Disorders
    Alexander J. Kastaniotis, Kaija J. Autio, Remya R. Nair
    The Neuroscientist.2021; 27(2): 143.     CrossRef
  • The effects of chronic cadmium exposure on Bufo gargarizans larvae: Histopathological impairment, gene expression alteration and fatty acid metabolism disorder in the liver
    Zongqi Ju, Jing Ya, Xinyi Li, Hongyuan Wang, Hongfeng Zhao
    Aquatic Toxicology.2020; 222: 105470.     CrossRef
  • Exploration of targets regulated by miR-125b in porcine adipocytes
    Xiao Cheng, Xingping Chen, Peng Wang, Ting Chen, Jiajie Sun, Qianyun Xi, Yongliang Zhang
    In Vitro Cellular & Developmental Biology - Animal.2020; 56(2): 103.     CrossRef
  • Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria
    Sara M Nowinski, Ashley Solmonson, Scott F Rusin, J Alan Maschek, Claire L Bensard, Sarah Fogarty, Mi-Young Jeong, Sandra Lettlova, Jordan A Berg, Jeffrey T Morgan, Yeyun Ouyang, Bradley C Naylor, Joao A Paulo, Katsuhiko Funai, James E Cox, Steven P Gygi,
    eLife.2020;[Epub]     CrossRef
  • Polymorphisms in ten candidate genes are associated with conformational and locomotive traits in Spanish Purebred horses
    Natalia Sevane, Susana Dunner, Ana Boado, Javier Cañon
    Journal of Applied Genetics.2017; 58(3): 355.     CrossRef
  • Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
    Chunhong Zhu, Weitao Song, Zhiyun Tao, Hongxiang Liu, Wenjuan Xu, Shuangjie Zhang, Huifang Li, Cristina Óvilo
    PLOS ONE.2017; 12(8): e0180403.     CrossRef
  • Mitochondrial fatty acid synthesis, fatty acids and mitochondrial physiology
    Alexander J. Kastaniotis, Kaija J. Autio, Juha M. Kerätär, Geoffray Monteuuis, Anne M. Mäkelä, Remya R. Nair, Laura P. Pietikäinen, Antonina Shvetsova, Zhijun Chen, J. Kalervo Hiltunen
    Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids.2017; 1862(1): 39.     CrossRef
  • Genetic modifications of Mecr reveal a role for mitochondrial 2-enoyl-CoA/ACP reductase in placental development in mice
    Remya R. Nair, Juha M. Kerätär, Kaija J. Autio, Ali J. Masud, Mikko A.J. Finnilä, Helena I. Autio-Harmainen, Ilkka J. Miinalainen, Pentti A. Nieminen, J. Kalervo Hiltunen, Alexander J. Kastaniotis
    Human Molecular Genetics.2017; 26(11): 2104.     CrossRef
  • MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder
    Gali Heimer, Juha M. Kerätär, Lisa G. Riley, Shanti Balasubramaniam, Eran Eyal, Laura P. Pietikäinen, J. Kalervo Hiltunen, Dina Marek-Yagel, Jeffrey Hamada, Allison Gregory, Caleb Rogers, Penelope Hogarth, Martha A. Nance, Nechama Shalva, Alvit Veber, Mic
    The American Journal of Human Genetics.2016; 99(6): 1229.     CrossRef
  • Genome‐wide association study with the risk of schizophrenia in a Korean population
    Lyoung Hyo Kim, Byung Lae Park, Hyun Sub Cheong, Suhg Namgoong, Ji On Kim, Jeong‐Hyun Kim, Joong‐Gon Shin, Chul Soo Park, Bong‐Jo Kim, Jae Won Kim, Ihn‐Geun Choi, Jaeuk Hwang, Hyoung Doo Shin, Sung‐Il Woo
    American Journal of Medical Genetics Part B: Neuropsychiatric Genetics.2016; 171(2): 257.     CrossRef
  • A global transcriptional analysis of Megalobrama amblycephala revealing the molecular determinants of diet-induced hepatic steatosis
    Dingdong Zhang, Kangle Lu, Guangzhen Jiang, Wenbin Liu, Zaijie Dong, Hongyan Tian, Xiangfei Li
    Gene.2015; 570(2): 255.     CrossRef
  • Articles in 'Endocrinology and Metabolism' in 2014
    Won-Young Lee
    Endocrinology and Metabolism.2015; 30(1): 47.     CrossRef
Close layer

Endocrinol Metab : Endocrinology and Metabolism
TOP