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Endocrinol Metab : Endocrinology and Metabolism


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Chang Gun Cho  (Cho CG) 1 Article
Influence of Vitamin D Deficiency on Progression of Experimental Otitis Media in Rats
Hee-Bok Kim, So Hyun Lim, Chang Gun Cho, Han Seok Choi
Endocrinol Metab. 2018;33(2):296-304.   Published online June 21, 2018
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Vitamin D plays an important role in the immune response against infection. The purpose of the present study was to investigate the influence of vitamin D deficiency on the progression of otitis media (OM) using an experimental rat model.


Four-week-old male Sprague-Dawley rats (n=72) were divided into two groups based on their diet: a control diet group (n=36) and a vitamin D-deficient diet group (n=36). After 8 weeks of diet, experimental OM was induced by inoculation of non-typeable Haemophilus influenzae in the middle ear cavity. The rats were evaluated with otomicroscopy to determine the inflammation in the middle ear mucosa on days 1, 2, 4, 7, and 14 post-inoculation. Bullae from sacrificed rats were collected and analyzed histologically.


The middle ear mucosa from rats with vitamin D deficiency showed a significantly higher thickness than that of controls during the course of OM. The maximum mucosal thickness was 56.0±9.1 µm in the vitamin D deficiency group, and 43.9±9.8 µm in the control group, although there was no significant difference in the tympanic membrane score between the two groups evaluated with otomicroscopy. An immunohistochemical study showed increased expression of interleukin 6 (IL-6) and tumor necrosis factor α in rats manifesting vitamin D deficiency and decreased expression of IL-10 compared with controls.


Our results showed that vitamin D deficiency may exacerbate the pathophysiological changes of OM via altered cytokine production. Therefore, maintaining vitamin D status in the optimal range may be beneficial for proper management of OM.


Citations to this article as recorded by  
  • The role of vitamin D in subjective tinnitus—A case-control study
    Magdalena Nowaczewska, Stanisław Osiński, Maria Marzec, Michał Wiciński, Katarzyna Bilicka, Wojciech Kaźmierczak, Rafael da Costa Monsanto
    PLOS ONE.2021; 16(8): e0255482.     CrossRef
  • Vitamin D and Otitis Media
    Rebecca E. Walker, Jim Bartley, Carlos A. Camargo, Edwin A. Mitchell
    Current Allergy and Asthma Reports.2019;[Epub]     CrossRef
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