- Diabetes, Obesity and Metabolism
- Role of TRPV4 Channel in Human White Adipocytes Metabolic Activity
-
Julio C. Sánchez, Aníbal Valencia-Vásquez, Andrés M. García
-
Endocrinol Metab. 2021;36(5):997-1006. Published online October 14, 2021
-
DOI: https://doi.org/10.3803/EnM.2021.1167
-
-
4,736
View
-
134
Download
-
6
Web of Science
-
6
Crossref
-
 Abstract
 PDF PubReader  ePub
- Background
Intracellular calcium (Ca2+) homeostasis plays an essential role in adipocyte metabolism and its alteration is associated with obesity and related disorders. Transient receptor potential vanilloid 4 (TRPV4) channels are an important Ca2+ pathway in adipocytes and their activity is regulated by metabolic mediators such as insulin. In this study, we evaluated the role of TRPV4 channels in metabolic activity and adipokine secretion in human white adipocytes.
Methods
Human white adipocytes were freshly cultured and the effects of the activation and inhibition of TRPV4 channels on lipolysis, glucose uptake, lactate production, and leptin and adiponectin secretion were evaluated.
Results
Under basal and isoproterenol-stimulated conditions, TRPV4 activation by GSK1016709A decreased lipolysis whereas HC067047, an antagonist, increased lipolysis. The activation of TRPV4 resulted in increased glucose uptake and lactate production under both basal conditions and insulin-stimulated conditions; in contrast HC067047 decreased both parameters. Leptin production was increased, and adiponectin production was diminished by TRPV4 activation and its inhibition had the opposite effect.
Conclusion
Our results suggested that TRPV4 channels are metabolic mediators involved in proadipogenic processes and glucose metabolism in adipocyte biology. TRPV4 channels could be a potential pharmacological target to treat metabolic disorders.
-
Citations
Citations to this article as recorded by  - Obesity, bone marrow adiposity, and leukemia: Time to act
Vijay Kumar, John H. Stewart
Obesity Reviews.2024;[Epub] CrossRef - TRP channels associated with macrophages as targets for the treatment of obese asthma
Wenzhao Zhu, Dinxi Bai, Wenting Ji, Jing Gao
Lipids in Health and Disease.2024;[Epub] CrossRef - Multidisciplinary Advances Address the Challenges in Developing Drugs against Transient Receptor Potential Channels to Treat Metabolic Disorders
Yibing Wang
ChemMedChem.2023;[Epub] CrossRef - Ion channels regulate energy homeostasis and the progression of metabolic disorders: Novel mechanisms and pharmacology of their modulators
Wenyi Wu, Jianan Zheng, Ru Wang, Yibing Wang
Biochemical Pharmacology.2023; 218: 115863. CrossRef - Potential lipolytic regulators derived from natural products as effective approaches to treat obesity
Xi-Ding Yang, Xing-Cheng Ge, Si-Yi Jiang, Yong-Yu Yang
Frontiers in Endocrinology.2022;[Epub] CrossRef - Adipokines: Deciphering the cardiovascular signature of adipose tissue
Joseph C. Galley, Shubhnita Singh, Wanessa M.C. Awata, Juliano V. Alves, Thiago Bruder-Nascimento
Biochemical Pharmacology.2022; 206: 115324. CrossRef
|
KES
